Cytotoxicity of seven recent dentine bonding agents on mouse 3T3 fibroblast cells

dc.contributor.authorOlivier, Annette
dc.contributor.authorGrobler, Sias Renier
dc.contributor.authorOsman, Yusuf Ismail
dc.date.accessioned2013-05-03T13:43:01Z
dc.date.available2013-05-03T13:43:01Z
dc.date.issued2012
dc.description.abstractToday it is generally accepted that most bonding agents are cytotoxic. In this study the relative cyto-toxicity of seven recent dentine bonding agents on mouse 3T3 fibroblast cells were investigated. Materials and Methods. Near-confluent mouse 3T3 fibro- blast cells were exposed to Dulbecco Modified Eagle’s Medium containing extractions from the seven different bonding agents. The cell survival rate was then determined using the standard MTT assay. Results. The cell survival rate ranking is: iBond (94%) < Gbond (78%) < Xeno V (71%) < Adper Easy Bond (63%) < Xeno V+ (61%) < Adper Scotchbond SE (33%) < XP Bond (32%). Part A of Adper Scotchbond SE had a survival rate of 35% and part B 38%. These two parts did not differ significantly. Adper Scotchbond SE and XP Bond do not differ significantly. While Xeno V+, Xeno V and Adper Easy Bond do not differ. (p < 5%; Tukey-Kramer Multiple-Comparison Test). Conclusion. All of the tested adhesive bonding agents were cytotoxic with survival rate of 3T3 cells between 94% to 31%. Of the 7 bonding agents tested iBond was found to be only slightly toxic and by far the least toxic. The two bonding agents (XP Bond and Adper Scotchbond SE) containing UDMA plus TEGDMA plus HEMA plus camphorquinone were found to be the most toxic.en_US
dc.identifier.citationOlivier, A., Grobler, S. & Osman, Y. (2012). Cytotoxicity of seven recent dentine bonding agents on mouse 3T3 fibroblast cells. Open Journal of Stomatology. 2: 244 – 250en_US
dc.identifier.issn2160-8717
dc.identifier.urihttp://hdl.handle.net/10566/589
dc.language.isoenen_US
dc.privacy.showsubmitterfalse
dc.publisherScientific Research Publishingen_US
dc.rights© 2012 Olivier et al; licensee Scientific Research Publishing. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.source.urihttp://dx.doi.org/10.4236/ojst.2012.24043
dc.status.ispeerreviewedtrue
dc.subjectCytotoxicityen_US
dc.subjectBonding Agentsen_US
dc.subjectMouse 3T3 Fibroblasten_US
dc.titleCytotoxicity of seven recent dentine bonding agents on mouse 3T3 fibroblast cellsen_US
dc.typeArticleen_US

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