In vitro acetylcholinesterase inhibitory activity and chemical composition of the dichloromethane extracts of the leaves and inflorescences of the tumbleweed, Trachyandra laxa (N.E.Br.) Oberm. var. rigida (Suesseng.) Roessl

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dc.contributor.authorVan Wyk, Hedwig M
dc.contributor.authorSchoeman, Dewald
dc.contributor.authorKwembeya, Ezekeil
dc.date.accessioned2022-02-07T09:31:50Z
dc.date.available2022-02-07T09:31:50Z
dc.date.issued2022
dc.description.abstractTrachyandra laxa, which belongs to the family Asphodelaceae, is a poisonous geophyte, often consumed by livestock, particularly when pastures are poor. Ingestion of T. laxa by livestock causes paresis and paralysis of the animals. However, the chemical composition of this plant has never been studied before and its toxic components are still unknown. In this study, an acetylcholinesterase (AChE) inhibition assay of different extracts of the parts of Trachyandra laxa (N.E.Br.) Oberm. var. rigida (Suesseng.) Roessl. revealed that the DCM extracts possess the highest AChE inhibitory activity. In addition, using qualitative phytochemical tests, it was determined that all the plant parts contain polyphenols and tannins, while only the leaves contain flavonoids. Saponins were detected in all plant parts, except in the flowers and anthraquinones were detected in all plant parts expect the roots. Using GC MS analysis, 13 volatile constituents were identified in the DCM extracts of the leaves and inflorescences, including a,b-unsaturated lactones, carboxylic acids and esters, a diterpenoid, phytol, and a phytosterol, b-sitosterol. Of particular interest are the a,b-unsaturated lactones, which were identified as dihydroactinidiolide (DHA) and its hydroxylated analogue, hydroxy‑DHA, as well as phytol, a diterpenoid. DHA and phytol have been reported to possess potent AChE inhibition activity. It is therefore plausible that these compounds and potentially also hydroxy‑DHA could contribute to the AChE inhibitory activity of the DCM extracts, and thus the neurotoxicity of T. laxa var. rigida.en_US
dc.identifier.citationvan Wyk, H. M., Schoeman, D., Kwembeya, E., Hans, R. H., Pool, E. J., & Louw, S. (2022). In vitro acetylcholinesterase inhibitory activity and chemical composition of the dichloromethane extracts of the leaves and inflorescences of the tumbleweed, Trachyandra laxa (N.E.Br.) Oberm. var. rigida (Suesseng.) Roessl. South African Journal of Botany, 147, 231–237. https://doi.org/10.1016/j.sajb.2021.12.013en_US
dc.identifier.urihttps://doi.org/10.1016/j.sajb.2021.12.013
dc.identifier.urihttp://hdl.handle.net/10566/7177
dc.language.isoenen_US
dc.publisherSouth African Journal of Botanyen_US
dc.subjectneurotoxicityen_US
dc.subjectlivestock poisoningen_US
dc.subjectacetylcholinesterase inhibitionen_US
dc.subjecta,b-unsaturated lactoneen_US
dc.subjectdihydroactinidiolideen_US
dc.subjectphytolen_US
dc.titleIn vitro acetylcholinesterase inhibitory activity and chemical composition of the dichloromethane extracts of the leaves and inflorescences of the tumbleweed, Trachyandra laxa (N.E.Br.) Oberm. var. rigida (Suesseng.) Roesslen_US
dc.typeArticleen_US

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