A plant-produced SARS-CoV-2 spike protein elicits heterologous immunity in hamsters

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dc.contributor.authorMargolin, Emmanuel
dc.contributor.authorSchäfer, Georgia
dc.contributor.authorShaw, Megan L.
dc.date.accessioned2023-04-21T09:36:13Z
dc.date.available2023-04-21T09:36:13Z
dc.date.issued2023
dc.description.abstractMolecular farming of vaccines has been heralded as a cheap, safe and scalable production platform. In reality, however, differences in the plant biosynthetic machinery, compared to mammalian cells, can complicate the production of viral glycoproteins. Remodelling the secretory pathway presents an opportunity to support key post-translational modifications, and to tailor aspects of glycosylation and glycosylation-directed folding. In this study, we applied an integrated host and glyco-engineering approach, NXS/T Generation™, to produce a SARS-CoV-2 prefusion spike trimer in Nicotiana benthamiana as a model antigen from an emerging virus. The size exclusion-purified protein exhibited a characteristic prefusion structure when viewed by transmission electron microscopy, and this was indistinguishable from the equivalent mammalian cell-produced antigen.en_US
dc.identifier.citationMargolin, E. et al. (2023). A plant-produced SARS-CoV-2 spike protein elicits heterologous immunity in hamsters. Frontiers in Plant Science, 14, 1146234. https://doi.org/10.3389/fpls.2023.1146234en_US
dc.identifier.issn1664-462X
dc.identifier.urihttps://doi.org/10.3389/fpls.2023.1146234
dc.identifier.urihttp://hdl.handle.net/10566/8855
dc.language.isoenen_US
dc.publisherFrontiers Mediaen_US
dc.subjectGlycoproteinen_US
dc.subjectGlycosylationen_US
dc.subjectVaccineen_US
dc.subjectBioscienceen_US
dc.subjectMedicineen_US
dc.titleA plant-produced SARS-CoV-2 spike protein elicits heterologous immunity in hamstersen_US
dc.typeArticleen_US

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