To explore the effect of prednisolone on blood-brain barrier endothelial cells treated with lipopolysaccharides.
| dc.contributor.author | Siebrits, Michaela Nicole | |
| dc.contributor.supervisor | Makhathini, Khayelihle Brain | |
| dc.date.accessioned | 2024-11-04T13:23:15Z | |
| dc.date.available | 2024-11-04T13:23:15Z | |
| dc.date.issued | 2024 | |
| dc.description.abstract | The blood-brain barrier (BBB) is primarily regulated by highly selective endothelial cells. Inflammation of the brain capillaries can compromise the BBB’s functioning. Methylprednisolone (M-Pred), a synthetic glucocorticoid, is generally prescribed to treat inflammation, though little is known of its effect on inflamed brain capillaries. This study evaluated the effectiveness of M-Pred in ameliorating the LPS-induced inflammatory response in the in vitro BBB model. Cultured brain endothelial cells (bEnd.3) were exposed to LPS (500, 1000, 2000 ng/ml) and co-treated with selected concentrations of M-Pred (10, 20, 40 μM). Cell viability, cell toxicity, and cell proliferation, bEnd.3 monolayer permeability (Transendothelial Electrical Resistance) were investigated, while ELISA kits were used to quantify pro-inflammatory cytokines, TNF-α and IL-1β. | |
| dc.identifier.uri | https://hdl.handle.net/10566/17328 | |
| dc.language.iso | en | |
| dc.publisher | Universty of the Western Cape | |
| dc.subject | Blood-brain barrier | |
| dc.subject | Inflammation | |
| dc.subject | Lipopolysaccharide | |
| dc.subject | Prednisolone | |
| dc.subject | Methylprednisolone | |
| dc.title | To explore the effect of prednisolone on blood-brain barrier endothelial cells treated with lipopolysaccharides. | |
| dc.type | Thesis |