Electrochemical immunosensor based on polythionine/gold nanoparticles for the determination of Aflatoxin B1

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dc.contributor.authorOwino, Joseph H.O.
dc.contributor.authorArotiba, Omotayo A.
dc.contributor.authorHendricks, Nicolette
dc.contributor.authorSonga, Everlyne
dc.contributor.authorJahed, Nazeem
dc.contributor.authorWaryo, Tesfaye T.
dc.contributor.authorNgece, Rachel F.
dc.contributor.authorBaker, Priscilla
dc.contributor.authorIwuoha, Emmanuel I.
dc.date.accessioned2016-12-21T12:55:16Z
dc.date.available2016-12-21T12:55:16Z
dc.date.issued2008
dc.description.abstractAn aflatoxin B1 (AFB1) electrochemical immunosensor was developed by the immobilisation of aflatoxin B1-bovine serum albumin (AFB1-BSA) conjugate on a polythionine (PTH)/gold nanoparticles (AuNP)-modified glassy carbon electrode (GCE). The surface of the AFB1-BSA conjugate was covered with horseradish peroxidase (HRP), in order to prevent non-specific binding of the immunosensors with ions in the test solution. The AFB1 immunosensor exhibited a quasi-reversible electrochemistry as indicated by a cyclic voltammetric (CV) peak separation (ΔEp) value of 62 mV. The experimental procedure for the detection of AFB1 involved the setting up of a competition between free AFB1 and the immobilised AFB1-BSA conjugate for the binding sites of free anti-aflatoxin B1 (anti-AFB1) antibody. The immunosensor’s differential pulse voltammetry (DPV) responses (peak currents) decreased as the concentration of free AFB1 increased within a dynamic linear range (DLR) of 0.6 - 2.4 ng/mL AFB1 and a limit of detection (LOD) of 0.07 ng/mL AFB1. This immunosensing procedure eliminates the need for enzyme-labeled secondary antibodies normally used in conventional ELISA–based immunosensors.en_US
dc.identifier.citationOwino, J.H.O. et al. (2008). Electrochemical immunosensor based on polythionine/gold nanoparticles for the determination of Aflatoxin B1. Sensors, 8(12), 8262-8274en_US
dc.identifier.issn1424-8220
dc.identifier.urihttp://hdl.handle.net/10566/2475
dc.identifier.urihttp://dx.doi.org/10.3390/s8128262
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAll articles published by MDPI are made immediately available worldwide under an open access license.
dc.subjectImmunosensoren_US
dc.subjectGold nanoparticlesen_US
dc.subjectAflatoxin B1en_US
dc.subjectPolythionineen_US
dc.subjectHorseradish peroxidise (HRP)en_US
dc.titleElectrochemical immunosensor based on polythionine/gold nanoparticles for the determination of Aflatoxin B1en_US
dc.typeArticleen_US

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