Frequency and voltage-modulated electrochemical aflatoxin B1 immunosensor systems prepared on electroactive organic polymer platforms

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dc.contributor.advisorBaker, P.G.L.
dc.contributor.advisorIwuoha, Emmanuel
dc.contributor.authorOwino, Joseph Hasael Odero
dc.contributor.otherDept. of Chemistry
dc.contributor.otherFaculty of Science
dc.date.accessioned2014-02-07T08:36:55Z
dc.date.accessioned2024-05-13T12:41:05Z
dc.date.available2010/04/30 03:02
dc.date.available2010/04/30
dc.date.available2014-02-07T08:36:55Z
dc.date.available2024-05-13T12:41:05Z
dc.date.issued2008
dc.descriptionPhilosophiae Doctor - PhDen_US
dc.description.abstractIn the presented work, immunosensors for detection of Aflatoxin B1 based on different immobilization platforms were studied. Synthesis of an electroactive hydrogel was also carried out. Aflatoxins are a group of mycotoxins that have deleterious effects on humans and are produced during fungal infection of plants or plant products. Electrochemical immunosensor for the determination of Aflatoxin B1 (AFB1) was developed with anti-aflatoxin B1 antibody immobilized on Pt electrodes modified with polyaniline (PANi) and polystyrene sulphonic acid (PSSA). Impedimetric analysis shows that the electron transfer resistances of Pt/PANi-PSSA electrode, Pt/PANi-PSSA/AFB1-Ab immunosensor and Pt/PANi-PSSA/AFB1-Ab incubated in BSA were 0.458, 720 and 1066 kΩ, respectively. These results indicate that electrochemical impedance spectroscopy (EIS) is a suitable method for monitoring the change in electron-transfer resistance associated with the immobilization of the antibody. Modelling of EIS data gave equivalent circuits which showed that the electron transfer resistance increased from 0.458 kΩ for Pt/PANi-PSSA electrode to 1066 kΩ for Pt/PANi-PSSA/AFB1-Ab immunosensor, indicating that immobilization of the antibody and incubation in BSA introduced an electron transfer barrier. The AFB1 immunosensor had a detection limit of 0.1 mg/L and a sensitivity of 869.6 kΩL/mg.en_US
dc.description.countrySouth Africa
dc.identifier.urihttps://hdl.handle.net/10566/14780
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.rights.holderUniversity of the Western Capeen_US
dc.subjectAflatoxin B1en_US
dc.subjectAnti-aflatoxin B1 antibodyen_US
dc.subjectImmunosensoren_US
dc.subjectElectrochemical impedance spectroscopyen_US
dc.subjectHydrogel composite Poly (2-hydroxyethyl methacrylate) p-(HEMA)en_US
dc.subjectGold nanoparticlesen_US
dc.subjectPolyanilineen_US
dc.subjectPoly thionineen_US
dc.titleFrequency and voltage-modulated electrochemical aflatoxin B1 immunosensor systems prepared on electroactive organic polymer platformsen_US
dc.typeThesisen_US

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Philosophiae Doctor - PhD (Chemistry)