Association between promoter methylation of MLH1 and MSH2 and reactive oxygen species in oligozoospermic men—A pilot study

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dc.contributor.authorGunes, S.
dc.contributor.authorAgarwal, A.
dc.contributor.authorHenkel, Ralf
dc.contributor.authorSharma, Rajan
dc.contributor.authorEsteves, S.C.
dc.contributor.authorAljowair, A.
dc.contributor.authorEmirzeoglu, D.
dc.contributor.authorAlkhani, A.
dc.contributor.authorPelegrini, L.
dc.contributor.authorJoumah, A.
dc.contributor.authorSabanegh, E.
dc.contributor.authorMahmutoglu, A.M.
dc.date.accessioned2017-12-13T12:19:17Z
dc.date.available2017-12-13T12:19:17Z
dc.date.issued2017
dc.description.abstractMLH1 and MSH2 are important genes for DNA mismatch repair and crossing over during meiosis and are implicated in male infertility. Therefore, the methylation patterns of the DNA mismatch repair genes MLH1 and MSH2 in oligozoospermic males were investigated. Ten oligozoospermic patients and 29 normozoospermic donors were analysed. Methylation profiles of the MLH1 and MSH2 promotors were analysed. In addition, sperm motility and seminal reactive oxygen species (ROS) were recorded. Receiver operating characteristic (ROC) analysis was conducted to determine the accuracy of the DNA methylation status of MLH1 and MSH2 to distinguish between oligozoospermic and normozoospermic men. In oligozoospermic men, MLH1 was significantly (p = .0013) more methylated compared to normozoospermic men. Additionally, there was a significant positive association (r = .384; p = .0159) between seminal ROS levels and MLH1 methylation. Contrary, no association between MSH2 methylation and oligozoospermia was found. ROC curve analysis for methylation status of MLH1 was significant (p = .0275) with an area under the curve of 61.1%, a sensitivity of 22.2% and a specificity of 100.0%. This pilot study indicates oligozoospermic patients have more methylation of MLH1 than normozoospermic patients. Whether hypermethylation of the MLH1 promoter plays a role in repairing relevant mismatches of sperm DNA strands in idiopathic oligozoospermia warrants further investigation.en_US
dc.identifier.citationGunes, S. et al. (2017). Association between promoter methylation of MLH1 and MSH2 and reactive oxygen species in oligozoospermic men—A pilot study. Andrologia. 2017 : e12903en_US
dc.identifier.issn1439-0272
dc.identifier.urihttp://dx.doi.org/10.1111/and.12903
dc.identifier.urihttp://hdl.handle.net/10566/3331
dc.language.isoenen_US
dc.privacy.showsubmitterFALSE
dc.publisherWileyen_US
dc.rightsThis is the author-version of the article published online at:
dc.status.ispeerreviewedTRUE
dc.subjectDNA CpG methylationen_US
dc.subjectMLH1 gene promoter regionen_US
dc.subjectMSH2 gene promoter regionen_US
dc.subjectOligozoospermic menen_US
dc.titleAssociation between promoter methylation of MLH1 and MSH2 and reactive oxygen species in oligozoospermic men—A pilot studyen_US
dc.typeArticleen_US

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