Intact HIV proviruses persist in children seven to nine years after initiation of antiretroviral therapy in the first year of life

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dc.contributor.authorJoseph, Kevin
dc.contributor.authorKatusiime, Mary Grace
dc.contributor.authorHalvas, Elias Konstantine
dc.date.accessioned2021-01-12T08:21:08Z
dc.date.available2021-01-12T08:21:08Z
dc.date.issued2020
dc.description.abstractIn adults starting antiretroviral therapy (ART) during acute infection, 2% of proviruses that persist on ART are genetically intact by sequence analysis. In contrast, a recent report in children treated early failed to detect sequence-intact proviruses. In another cohort of children treated early, we sought to detect and characterize proviral sequences after 6 to 9 years on suppressive ART. Peripheral blood mononuclear cells (PBMC) from perinatally infected children from the Children with HIV Early antiRetroviral (CHER) study were analyzed. Nearly full-length proviral amplification and sequencing (NFL-PAS) were performed at one time point after 6 to 9 years on ART. Amplicons with large internal deletions were excluded (<9 kb). All amplicons of ≥9 kb were sequenced and analyzed through a bioinformatic pipeline to detect indels, frameshifts, or hypermutations that would render them defective. In eight children who started ART at a median age of 5.4 months (range, 2.0 to 11.1 months), 733 single NFL-PAS amplicons were generated. Of these, 534 (72.9%) had large internal deletions, 174 (23.7%) had hypermutations, 15 (1.4%) had small internal deletions, 3 (1.0%) had deletions in the packaging signal/major splice donor site, and 7 (1.0%) were sequence intact.en_US
dc.identifier.citationJoseph, K. et al. (2020). Intact HIV proviruses persist in children seven to nine years after initiation of antiretroviral therapy in the first year of life. Journal of Virology ,94(4),e01519-19en_US
dc.identifier.issn1098-5514
dc.identifier.uri10.1128/JVI.01519-19
dc.identifier.urihttp://hdl.handle.net/10566/5644
dc.language.isoenen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.subjectHIV cureen_US
dc.subjectHIV early ART childrenen_US
dc.subjectHIV full-length sequencingen_US
dc.subjectHIV intact provirusen_US
dc.subjectHIV persistenceen_US
dc.titleIntact HIV proviruses persist in children seven to nine years after initiation of antiretroviral therapy in the first year of lifeen_US
dc.typeArticleen_US

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