Development of amperometric biosensor with cyclopentadienylruthenium (II) thiolato schiff base self-assembled monolayer (SAM) on gold

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dc.contributor.advisorBaker, Priscilla
dc.contributor.advisorIwuoha, Emmanuel
dc.contributor.authorTicha, Lawrence Awa
dc.contributor.otherDept. of Chemistry
dc.contributor.otherFaculty of Science
dc.date.accessioned2013-11-16T09:21:44Z
dc.date.accessioned2024-05-09T10:51:30Z
dc.date.available2012/07/03 14:44
dc.date.available2012/07/03
dc.date.available2013-11-16T09:21:44Z
dc.date.available2024-05-09T10:51:30Z
dc.date.issued2007
dc.descriptionMagister Scientiae - MScen_US
dc.description.abstractA novel cyclopentadienylruthenium(II) thiolato Schiff base, [Ru(SC6H4NC(H)C6H4OCH2CH2SMe)(η5-C2H5]2 was synthesized and deposited as a selfassembled monolayer (SAM) on a gold electrode. Effective electronic communication between the Ru(II) centers and the gold electrode was established by electrostatically cycling the Shiff base-doped gold electrode in 0.1 M NaOH from -200 mV to +600 mV. The SAMmodified gold electrode (Au/SAM) exhibited quasi-reversible electrochemistry. The integrity of this electro-catalytic SAM, with respect to its ability to block and electro-catalyze certain Faradaic processes, was interrogated using Cyclic and Osteryoung Square Wave voltammetric experiments. The formal potential, E0', varied with pH to give a slope of about - 34 mV pH-1. The surface concentration, Γ, of the ruthenium redox centers was found to be 1.591 x 10-11 mol cm-2. By electrostatically doping the Au/SAM/Horseradish peroxidase at an applied potential of +700 mV vs Ag/AgCl, a biosensor was produced for the amperometric analysis of hydrogen peroxide, cumene hydroperoxide and tert-butylhydroperoxide. The electrocatalytic-type biosensors displayed typical Michaelis-Menten kinetics with their limits of detection of 6.45 M, 6.92 M and 7.01 M for hydrogen peroxide, cumene hydroperoxide and tert-butylhydroperoxide respectively.en_US
dc.description.countrySouth Africa
dc.identifier.urihttps://hdl.handle.net/10566/14608
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.rights.holderUniversity of the Western Capeen_US
dc.subjectSelf-assembled monolayer (SAM)en_US
dc.subjectCyclopentadienylruthenium(II) thiolato Shiff baseen_US
dc.subjectBiosensorsen_US
dc.subjectElectrostatic dopingen_US
dc.subjectHorseradish peroxidase Peroxidesen_US
dc.subjectCyclic Voltammetry (CV)en_US
dc.subjectOsteryoung Square Wave voltammetry (OSWV)en_US
dc.titleDevelopment of amperometric biosensor with cyclopentadienylruthenium (II) thiolato schiff base self-assembled monolayer (SAM) on golden_US
dc.typeThesisen_US

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