Pineapple stem-derived bromelain based priming improves pepper seed protein reserve mobilization, germination, emergence and plant growth

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Date

2021

Journal Title

Journal ISSN

Volume Title

Publisher

Springer

Abstract

Pepper seeds are slow to germinate and emergence is often non-uniform and incomplete, reducing gains from this cash crop. This study investigated the effects of pineapple stem- derived protease (stem bromelain) based priming on pepper seed germination in relation to reserve mobilization (specifically, proteins and amino acids), germination, emergence and plant growth. These parameters were compared across two controls, (1) unsoaked seeds and (2) seeds soaked in deionized water, and seeds soaked in pineapple stem bromelain crude extract (treatment). Seeds were soaked in bromelain crude extract possessing a proteolytic activity of 6.25 tU or deionized water (first control) for 3 h at 35 C. Light microscopy revealed an abundance of protein bodies in the endosperm of the seeds prior to imbibition.

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Keywords

Ananas comosus, Capsicum annuum, Plants, Natural products, Biology, Biodiversity

Citation

Perez, L. et al. (2021). Pineapple stem-derived bromelain based priming improves pepper seed protein reserve mobilization, germination, emergence and plant growth. Physiology and Molecular Biology of Plants, 27(8), 1651–1657. https://doi.org/10.1007/s12298-021-01038-7