PCR-based detection of non-indigenous microorganisms in ‘pristine’ environments

dc.contributor.authorBaker, Gillian
dc.contributor.authorAh Tow, Lemese
dc.contributor.authorCowan, Donald A.
dc.date.accessioned2010-09-27T12:28:05Z
dc.date.available2010-09-27T12:28:05Z
dc.date.issued2003
dc.description.abstractPCR-based technologies are widely employed for the detection of specific microorganisms, and may be applied to the identification of non-indigenous microorganisms in ‘pristine’ environments. For ‘pristine’ environments such as those found on the Antarctic continent, the application of these methods to the assessment of environmental contamination from human activities must be treated with caution. Issues such as the possibility of non-human dispersal of organisms, stability and survival of non-indigenous organisms in vivo, the sensitivity, reproducibility and specificity of the PCR process (and particularly primer design) and the sampling regime employed must all be considered in detail. We conclude that despite these limitations, PCR and related technologies offer enormous scope for assessment of both natural and non-indigenous microbial distributions.en_US
dc.identifier.citationBaker, G., Ah Tow, L., Cowan, D.A. (2003). PCR-based detection of non-indigenous micro-organisms in ‘pristine’ environments. J. Microbiol. Methods. 53:157-164.en_US
dc.identifier.urihttp://hdl.handle.net/10566/149
dc.inquiriesdcowan@uwc.ac.za
dc.language.isoenen_US
dc.privacy.showsubmittertrue
dc.publisherElsevieren_US
dc.rightsThis is the author post-print of an article published by Elsevier Science B.V.
dc.source.urihttp://dx.doi.org/10.1016/S0167-7012(03)00021-6
dc.status.ispeerreviewedtrue
dc.subjectAntarcticaen_US
dc.subjectEndemismen_US
dc.subjectHuman commensalsen_US
dc.subjectPsychrophileen_US
dc.subjectPrimer designen_US
dc.titlePCR-based detection of non-indigenous microorganisms in ‘pristine’ environmentsen_US
dc.typeArticleen_US

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