Odorant and gustatory receptors in the tsetse fly Glossina morsitans morsitans

dc.contributor.authorObiero, George F.
dc.contributor.authorNyanjom, Steven R. G.
dc.contributor.authorMireji, Paul O.
dc.contributor.authorChristoffels, Alan
dc.contributor.authorRobertson, Hugh M.
dc.contributor.authorMasiga, Daniel K.
dc.date.accessioned2015-06-09T14:34:11Z
dc.date.available2015-06-09T14:34:11Z
dc.date.issued2014
dc.description.abstractTsetse flies use olfactory and gustatory responses, through odorant and gustatory receptors (ORs and GRs), to interact with their environment. Glossina morsitans morsitans genome ORs and GRs were annotated using homologs of these genes in Drosophila melanogaster and an ab initio approach based on OR and GR specific motifs in G. m. morsitans gene models coupled to gene ontology (GO). Phylogenetic relationships among the ORs or GRs and the homologs were determined using Maximum Likelihood estimates. Relative expression levels among the G. m. morsitans ORs or GRs were established using RNA-seq data derived from adult female fly. Overall, 46 and 14 putative G. m. morsitans ORs and GRs respectively were recovered. These were reduced by 12 and 59 ORs and GRs respectively compared to D. melanogaster. Six of the ORs were homologous to a single D. melanogaster OR (DmOr67d) associated with mating deterrence in females. Sweet taste GRs, present in all the other Diptera, were not recovered in G. m. morsitans. The GRs associated with detection of CO2 were conserved in G. m. morsitans relative to D. melanogaster. RNA-sequence data analysis revealed expression of GmmOR15 locus represented over 90% of expression profiles for the ORs. The G. m. morsitans ORs or GRs were phylogenetically closer to those in D. melanogaster than to other insects assessed. We found the chemoreceptor repertoire in G. m. morsitans smaller than other Diptera, and we postulate that this may be related to the restricted diet of blood-meal for both sexes of tsetse flies. However, the clade of some specific receptors has been expanded, indicative of their potential importance in chemoreception in the tsetse.en_US
dc.description.accreditationWeb of Scienceen_US
dc.description.sponsorshipGerman Academic Exchange Service (DAAD) South African Research Chair Initiative Department of Science and Technology National Research Foundation of South Africaen_US
dc.identifier.citationObiero G.F.O., et al. (2014) Odorant and gustatory receptors in the tsetse fly glossina morsitans morsitans. PLoS Neglected Tropical Diseases 8(4): e2663. doi:10.1371/journal.pntd.0002663en_US
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10566/1500
dc.language.isoenen_US
dc.privacy.showsubmitterfalse
dc.publisherPLOS
dc.rights© 2014 Obiero et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.source.urihttp://dx.doi.org/10.1371/journal.pntd.0002663
dc.status.ispeerreviewedtrue
dc.subjectDrosophila melanogasteren_US
dc.subjectTsetse flyen_US
dc.subjectChemoreceptionen_US
dc.titleOdorant and gustatory receptors in the tsetse fly Glossina morsitans morsitansen_US
dc.typeArticleen_US

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