The effects of endocrine disrupting chemicals on biomarkers of inflammation produced by lipopolysaccharide stimulated raw264.7 macrophages

Simple item page
dc.contributor.authorMakene, Vedastus W.
dc.contributor.authorPool, Edmund J.
dc.date.accessioned2023-02-13T10:57:19Z
dc.date.available2023-02-13T10:57:19Z
dc.date.issued2019
dc.description.abstractEndocrine disrupting chemicals (EDCs) are common pollutants in the environment and can induce disruption of the endocrine and immune systems. The present study evaluated the effects of selected common environmental EDCs on secretion of inflammatory biomarkers by RAW264.7 cells. The EDCs investigated were Estradiol (E2), 5α-dihydrotestosterone (DHT), and Bisphenol A (BPA). To evaluate if the effects caused by EDCs were modulated by steroid hormone receptors, antagonists of estrogen and androgen receptors were used. The steroid receptor antagonists used were Tamoxifen, an estrogen receptor antagonist, and Flutamide, an androgen receptor antagonist. Secretion of biomarkers of inflammation, namely nitric oxide (NO) and interleukin 6 (IL-6), were monitored. The NO was determined using Griess reaction and IL-6 was measured by enzyme linked immunosorbent assay (ELISA). Although 5 µg/mL E2, DHT, and BPA were not toxic to RAW264.7 cell cultures, the same treatments significantly (p < 0.001) reduced both NO and IL-6 secretion by lipopolysaccharide (LPS)-stimulated RAW264.7 cell cultures.en_US
dc.identifier.citationMakene, V. W., & Pool, E. J. (2019). The effects of endocrine disrupting chemicals on biomarkers of inflammation produced by lipopolysaccharide stimulated raw264.7 macrophages. International Journal of Environmental Research and Public Health, 16(16), 2914. https://doi.org/10.3390/ijerph16162914en_US
dc.identifier.issn1660-4601
dc.identifier.urihttps://doi.org/10.3390/ijerph16162914
dc.identifier.urihttp://hdl.handle.net/10566/8422
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectBioscienceen_US
dc.subjectNitric oxideen_US
dc.subjectAnti-inflammatoryen_US
dc.subjectMedicineen_US
dc.titleThe effects of endocrine disrupting chemicals on biomarkers of inflammation produced by lipopolysaccharide stimulated raw264.7 macrophagesen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
makene_the effects of endocrine disrupting chemicals_2019.pdf
Size:
729.54 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Research Articles (Medical Bioscience)