A low-cost flow cytometric assay for the detection and quantification of apoptosis using an anionic halogenated fluorescein dye

dc.contributor.authorMeyer, Mervin
dc.contributor.authorEssack, Magbubah
dc.contributor.authorKanyanda, Stonard
dc.date.accessioned2023-03-08T08:36:40Z
dc.date.available2023-03-08T08:36:40Z
dc.date.issued2008
dc.description.abstractWe describe here a technical improvement of an established colorimetric method used to detect and measure the occurrence of apoptosis in mammalian cells during in vitro cell culture. This assay uses an anionic halogenated fluorescein dye that is taken up by apoptotic cells at the stage of phosphatidylserine externalization. We demonstrate that apoptotic cells stained with this dye can be detected by flow cytometric analysis. Furthermore, we show that the modified method compares well with the standard annexin-V–based apoptosis assay and that it is significantly more cost-effective than the annexin-V assay.en_US
dc.identifier.citationMeyer, M et al. (2008). A low-cost flow cytometric assay for the detection and quantification of apoptosis using an anionic halogenated fluorescein dye. BioTechniques, 45 (3) ,317-320. https://doi.org/10.2144/000112908en_US
dc.identifier.issn1940-9818
dc.identifier.urihttps://doi.org/10.2144/000112908
dc.identifier.urihttp://hdl.handle.net/10566/8550
dc.language.isoenen_US
dc.publisherFuture Science Groupen_US
dc.subjectBiologyen_US
dc.subjectTechnologyen_US
dc.subjectBioscienceen_US
dc.subjectScienceen_US
dc.titleA low-cost flow cytometric assay for the detection and quantification of apoptosis using an anionic halogenated fluorescein dyeen_US
dc.typeArticleen_US

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