A low-cost flow cytometric assay for the detection and quantification of apoptosis using an anionic halogenated fluorescein dye
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Date
2008
Journal Title
Journal ISSN
Volume Title
Publisher
Future Science Group
Abstract
We describe here a technical improvement of an established colorimetric method used to detect and measure the occurrence of apoptosis in mammalian cells during in vitro cell culture. This assay uses an anionic halogenated fluorescein dye that is taken up by apoptotic cells at the stage of phosphatidylserine externalization. We demonstrate that apoptotic cells stained with this dye can be detected by flow cytometric analysis. Furthermore, we show that the modified method compares well with the standard annexin-V–based apoptosis assay and that it is significantly more cost-effective than the annexin-V assay.
Description
Keywords
Biology, Technology, Bioscience, Science
Citation
Meyer, M et al. (2008). A low-cost flow cytometric assay for the detection and quantification of apoptosis using an anionic halogenated fluorescein dye. BioTechniques, 45 (3) ,317-320. https://doi.org/10.2144/000112908