Increasing extracellular cellulase activity of the recombinant Saccharomyces cerevisiae by engineering cell wall-related proteins for improved consolidated processing of carbon neutral lignocellulosic biomass
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Date
2022
Authors
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Publisher
Elsevier
Abstract
Sustainable bioproduction using carbon neutral feedstocks, especially lignocellulosic biomass, has attracted
increasing attention due to concern over climate change and carbon reduction. Consolidated bioprocessing (CBP)
of lignocellulosic biomass using recombinant yeast of Saccharomyces cerevisiae is a promising strategy for lignocellulosic biorefinery. However, the economic viability is restricted by low enzyme secretion levels. For
more efficient CBP, MIG1 spsc01 isolated from the industrial yeast which encodes the glucose repression regulator
derivative was overexpressed. Increased extracellular cellobiohydrolase (CBH) activity was observed with unexpectedly decreased cell wall integrity. Further studies revealed that disruption of CWP2, YGP1, and UTH1,
which are functionally related to MIG1 spsc01, also enhanced CBH secretion. Subsequently, improved cellulase
production was achieved by simultaneous disruption of YGP1 and overexpression of SED5, which remarkably
increased extracellular CBH activity of 2.2-fold over the control strain. These results provide a novel strategy to
improve the CBP yeast for bioconversion of carbon neutral biomass.
Description
Keywords
Biotechnology, Cellulase production, Climate change, Waste management
Citation
Li, J. et al. (2022). Increasing extracellular cellulase activity of the recombinant Saccharomyces cerevisiae by engineering cell wall-related proteins for improved consolidated processing of carbon neutral lignocellulosic biomass. Bioresource Technology, 365, 128132. https://doi.org/10.1016/j.biortech.2022.128132