Recent advances in the detection of interferon‑gamma as a TB biomarker
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Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
Abstract
Tuberculosis (TB) is one of the main infectious diseases worldwide and accounts for many deaths. It is caused by Mycobacterium tuberculosis usually afecting the lungs of patients. Early diagnosis and treatment are essential to control the TB
epidemic. Interferon-gamma (IFN-γ) is a cytokine that plays a part in the body’s immune response when fghting infection.
Current conventional antibody-based TB sensing techniques which are commonly used include enzyme-linked immunosorbent assay (ELISA) and interferon-gamma release assays (IGRAs). However, these methods have major drawbacks,
such as being time-consuming, low sensitivity, and inability to distinguish between the diferent stages of the TB disease.
Several electrochemical biosensor systems have been reported for the detection of interferon-gamma with high sensitivity
and selectivity. Microfuidic techniques coupled with multiplex analysis in regular format and as lab-on-chip platforms have
also been reported for the detection of IFN-γ. This article is a review of the techniques for detection of interferon-gamma
as a TB disease biomarker. The objective is to provide a concise assessment of the available IFN-γ detection techniques
(including conventional assays, biosensors, microfuidics, and multiplex analysis) and their ability to distinguish the diferent stages of the TB disease.
Description
Keywords
Aptasensor, Biosensor, Cytokine, Nanoscience and nanotechnology, Tuberculosis
Citation
Januarie, K. C.et al. (2021). Recent advances in the detection of interferon‑gamma as a TB biomarker. Analytical and Bioanalytical Chemistry. https://doi.org/10.1007/s00216-021-03702-z