Genomic characterization of a prophage, Smhb1, that Infects Salinivibrio kushneri BNH isolated from a Namib Desert saline spring

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dc.contributor.authorOlonade, Israel
dc.contributor.authorvan Zyl, Leonardo Joaquim
dc.contributor.authorTrindade, Marla
dc.date.accessioned2021-10-08T09:13:37Z
dc.date.available2021-10-08T09:13:37Z
dc.date.issued2021
dc.descriptionFunding text 1 Funding: This work was supported by the National Research Foundation (NRF) of South Africa. Funding text 2 This work was supported by the National Research Foundation (NRF) of South Africa. We wish to thank the researcher and management of the Gobabeb Desert research station for hosting us during sample collection. We also thank Mohammed Jaffer at the Electron Microscope Unit at the University of Cape Town for helping to capture TEM images.en_US
dc.description.abstractRecent years have seen the classification and reclassification of many viruses related to the model enterobacterial phage P2. Here, we report the identification of a prophage (Smhb1) that infects Salinivibrio kushneri BNH isolated from a Namib Desert salt pan (playa). Analysis of the genome revealed that it showed the greatest similarity to P2-like phages that infect Vibrio species and showed no relation to any of the previously described Salinivibrio-infecting phages. Despite being distantly related to these Vibrio infecting phages and sharing the same modular gene arrangement as seen in most P2-like viruses, the nucleotide identity to its closest relatives suggest that, for now, Smhb1 is the lone member of the Peduovirus genus Playavirus. Although host range testing was not extensive and no secondary host could be identified for Smhb1, genomic evidence suggests that the phage is capable of infecting other Salinivibrio species, including Salinivibrio proteolyticus DV isolated from the same playa. Taken together, the analysis presented here demonstrates how adaptable the P2 phage model can be. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.en_US
dc.description.sponsorshipNational Research Foundation (NRF University of Cape Town (UCT)en_US
dc.identifier.citationOlonade, I., van Zyl, L.J., & Trindade, M. (2021). Genomic Characterization of a Prophage, Smhb1, That Infects Salinivibrio kushneri BNH Isolated from a Namib Desert Saline Spring. Microorganisms. 9. 2043. DOI: 10.3390/microorganisms9102043.en_US
dc.identifier.issn20762607
dc.identifier.uri10.3390/microorganisms9102043
dc.identifier.urihttp://hdl.handle.net/10566/6873
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectMyoviridaeen_US
dc.subjectNamiben_US
dc.subjectPhageen_US
dc.subjectPlayaen_US
dc.subjectSalinivibrioen_US
dc.subjectModel enterobacterial phage P2en_US
dc.subjectVirusesen_US
dc.subjectVibrio speciesen_US
dc.subjectGenome analysisen_US
dc.subjectNamibiaen_US
dc.subjectSalt pansen_US
dc.titleGenomic characterization of a prophage, Smhb1, that Infects Salinivibrio kushneri BNH isolated from a Namib Desert saline springen_US
dc.typeArticleen_US

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