Characterisation of the arsenic resistance genes in Bacillus sp. UWC isolated from maturing fly ash acid mine drainage neutralised solids

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dc.contributor.authorMusingarimi, Wicleffe
dc.contributor.authorTuffin, Marla
dc.contributor.authorCowan, Donald A.
dc.date.accessioned2010-10-19T14:18:12Z
dc.date.available2010-10-19T14:18:12Z
dc.date.issued2010
dc.description.abstractAn arsenic resistant Bacillus sp. UWC was isolated from fly ash acid mine drainage (FA-AMD) neutralised solids. A genomic library was prepared and screened in an arsenic sensitive mutant Escherichia coli strain for the presence of arsenic resistance (ars) genes. Sequence analysis of a clone conferring resistance to both sodium arsenite and sodium arsenate revealed homologues to the arsR (regulatory repressor), arsB (membrane located arsenite pump), arsC (arsenate reductase), arsD (second regulatory repressor and a metallochaperone) and arsA (ATPase) genes from known arsenic resistance operons. The Bacillus sp. UWC arsRBCDA genes were shown to be arranged in an unusual manner with the arsDA genes immediately downstream of arsC.en_US
dc.identifier.citationMusingarimi, W., Tuffin, M., & Cowan, D.A. (2010). Characterisation of the arsenic resistance genes in Bacillus sp. UWC isolated from maturing fly ash acid mine drainage neutralised solids. South African Journal of Science, 106 (1/2), Article #17: 5 pages.en_US
dc.identifier.urihttp://hdl.handle.net/10566/160
dc.inquiriesituffin@uwc.ac.za
dc.language.isoenen_US
dc.privacy.showsubmittertrue
dc.publisherAcademy of Science of South Africaen_US
dc.rights© 2010. The Authors.This work is licensed under the Creative Commons Attribution License.
dc.source.urihttp://dx.doi.org/10.4102/sajs. v106i1/2.17
dc.status.ispeerreviewedtrue
dc.subjectAcid mine drainageen_US
dc.subjectArsenicen_US
dc.subjectFly ashen_US
dc.subjectBacillusen_US
dc.subjectArsenic resistance genesen_US
dc.subjectGround water contaminationen_US
dc.titleCharacterisation of the arsenic resistance genes in Bacillus sp. UWC isolated from maturing fly ash acid mine drainage neutralised solidsen_US
dc.typeArticleen_US

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