HPLC-MS identification and expression of Candida drug-resistance proteins from African HIV-infected patients

Simple item page
dc.contributor.authorAbrantes, Pedro Miguel dos Santos
dc.contributor.authorFisher, Randall
dc.contributor.authorBouic, Patrick J D
dc.contributor.authorMcArthur, Carole P
dc.contributor.authorFielding, Burtram C
dc.contributor.authorAfrica, Charlene Wilma Joyce
dc.date.accessioned2021-09-27T07:55:42Z
dc.date.available2021-09-27T07:55:42Z
dc.date.issued2021-09-10
dc.description.abstractThe objective of this study was to elucidate the proteomic mechanisms of drug resistance in HIV-infected African patients. Cell membrane fractions from forty oral Candida isolates isolated from African HIV-positive patients were analysed using HPLC-MS with the aim of identifying proteins associated with their pathogenicity and drug resistance. Heat shock proteins that mediate the fungicidal activity of salivary peptides were found in all tested Candida fractions, with pH-responsive proteins associated with increased pathogenicity only being present in the three most commonly isolated species. ABC multidrug transporter efflux pumps and estrogen binding proteins were only found in C. albicans fractions, while ergosterol biosynthesis proteins were identified in four species. The combination of various adherence, invasion, upregulation and efflux pump mechanisms appear to be instrumental for the Candida host colonization and drug resistance emergence in HIV-infected individuals.en_US
dc.description.sponsorshipThis material is based upon work partially supported financially by the National Research Foundation of South Africa [Grant number TTK2008052700013].en_US
dc.identifier.citationPedro M D S Abrantes, Randall Fisher, Patrick J D Bouic, Carole P McArthur, Burtram C Fielding, Charlene W J Africa. HPLC-MS identification and expression of Candida drug-resistance proteins from African HIV-infected patients. AIMS Microbiology, 2021, 7(3): 320-335.en_US
dc.identifier.otherdoi: 10.3934/microbiol.2021020
dc.identifier.urihttp://hdl.handle.net/10566/6811
dc.language.isoenen_US
dc.publisherAIMS Pressen_US
dc.subjectCandidaen_US
dc.subjectDrug resistanceen_US
dc.subjectHPLC-MSen_US
dc.subjectProtein expressionen_US
dc.subjectHIVen_US
dc.titleHPLC-MS identification and expression of Candida drug-resistance proteins from African HIV-infected patientsen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
P Abrantes R Fisher P Bouic et al AIMS Microbiol Publication.pdf
Size:
523.69 KB
Format:
Adobe Portable Document Format
Description:
Main article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Research Articles (Medical Bioscience)