Proteomics of drug-resistant HIV-associated candidiasis

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dc.contributor.authorAbrantes, Pedro Miguel dos Santos
dc.contributor.authorBouic, Patrick J.D.
dc.contributor.authorAfrica, Charlene W.J.
dc.date.accessioned2015-09-30T11:39:06Z
dc.date.available2015-09-30T11:39:06Z
dc.date.issued2015
dc.description.abstractCandidiasis and HIV co-infection may cause increased patient morbidity and mortality due to oropharyngeal or systemic dissemination. Limited information exists on the prevalence, antifungal susceptibility profiles and drug resistance mechanisms of Candida species on the African continent, the highest HIV-affected region globally and home to new and emerging drug resistant Candida species. Candida species isolated from the oral mucosa of HIV-positive African patients were found to be resistant to many of the antifungals routinely used in HIV-associated candidiasis. Candida cell membrane fractions were examined using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and high performance liquid chromatography mass spectrometry (HPLC/MS) in order to elucidate the cell membrane proteins specifically expressed by antifungal drug resistant isolates. SDS-PAGE and HPLC/MS allowed for the identification of multi-drug resistance efflux transporter CDR2 proteins and the elucidation of Candida colonization mechanisms and pH-responsive proteins, with significant associations observed between specific drug resistance and the duration of antiretroviral (ARV) therapy. This study provided useful information on the mechanisms of antifungal resistance in Candida species. It also formed the basis for further studies to address the transfer of resistance between Candida species in an oral microbial biofilm.en_US
dc.identifier.citationAbrantes, P.M. et al. (2015). Proteomics of drug-resistant HIV-associated candidiasis. Poster presented at the 55th Interscience Conference on Antimicrobial Agents and Chemotherapy and 29th International Congress of Chemotherapy and Infection, at San Diego, California, U.S.A.en_US
dc.identifier.urihttp://hdl.handle.net/10566/1690
dc.language.isoenen_US
dc.privacy.showsubmitterfalse
dc.publisherUniversity of the Western Capeen_US
dc.rightsUniversity of the Western Cape
dc.status.ispeerreviewedtrue
dc.subjectCandidaen_US
dc.subjectHIVen_US
dc.subjectDrug resistanceen_US
dc.subjectProteomicsen_US
dc.subjectHIV co-infectionen_US
dc.subjectAfricaen_US
dc.subjectHIV-positive African patientsen_US
dc.subjectAntifungal resistanceen_US
dc.subjectAntiretroviral therapyen_US
dc.subjectSodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE)en_US
dc.subjectHigh performance liquid chromatography mass spectrometry (HPLC/MS)en_US
dc.titleProteomics of drug-resistant HIV-associated candidiasisen_US
dc.typePosteren_US

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