Cytochrome C biosensor for the determination of trace level arsenic and cyanide compounds

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dc.contributor.advisorBaker, Priscilla G.L.
dc.contributor.advisorIwuoha, Emmanuel
dc.contributor.authorFuku, Xolile Godfrey
dc.contributor.otherDept. of Chemistry
dc.contributor.otherFaculty of Science
dc.date.accessioned2013-08-02T11:48:11Z
dc.date.accessioned2024-05-09T10:49:57Z
dc.date.available2011/10/28 15:07
dc.date.available2011/10/28
dc.date.available2013-08-02T11:48:11Z
dc.date.available2024-05-09T10:49:57Z
dc.date.issued2011
dc.descriptionMagister Scientiae - MScen_US
dc.description.abstractIn this work, an electrochemical method based on a cyt c biosensor has been developed, for the detection of selected arsenic and cyanide compounds. Boron Doped Diamond (BDD) electrode was used as a transducer, onto which cyt c was immobilised and used for direct determination of Prussian blue, potassium cyanide and arsenic trioxide by inhibition mechanism. The sensitivity as calculated from cyclic voltammetry (CV) and square wave voltammetry (SWV), for each analyte in phosphate buffer (pH= 7) was found to be (1.087- 4.488 ×10-9 M) and the detection limits ranging from 0.0043- 9.1 μM. These values represent a big improvement over the current Environmental Protection Agency (EPA) and World Health Organisation (WHO) guidelines.en_US
dc.description.countrySouth Africa
dc.identifier.urihttps://hdl.handle.net/10566/14442
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.rights.holderUniversity of the Western Capeen_US
dc.subjectCytochrome c (cyt c)en_US
dc.subjectBoron doped diamond (BDD)en_US
dc.subjectPrussian blue (PB)en_US
dc.subjectArsenic trioxide (As)en_US
dc.subjectIncubationen_US
dc.subjectDrop coatingen_US
dc.subjectIntoxicationen_US
dc.subjectToxicityen_US
dc.subjectElectron-transport chainen_US
dc.subjectAsphyxiatesen_US
dc.subjectCyclic voltammetry (CV)en_US
dc.subjectSquare wave voltammetry (SWV)en_US
dc.subjectElectrochemical impedance spectroscopy (EIS)en_US
dc.subjectFourier Transform Infrared Spectroscopy (FTIR)en_US
dc.subjectUV/vis spectroscopyen_US
dc.subjectSubtractive Normalization Fourier Transform Infrared Spectroscopy (SNFTIR)en_US
dc.titleCytochrome C biosensor for the determination of trace level arsenic and cyanide compoundsen_US
dc.typeThesisen_US

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