Cellular Characterization of SARS Coronavirus Nucleocapsid

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dc.contributor.authorGoh, Phuay-Yee
dc.contributor.authorChoi, Yook-Wah
dc.contributor.authorShen, Shuo
dc.contributor.authorTan, Yee-Joo
dc.contributor.authorFielding, Burtram C.
dc.contributor.authorTan, Timothy H.P.
dc.contributor.authorO, Eng-Eong
dc.contributor.authorLim, Seng Gee
dc.contributor.authorHong, Wanjin
dc.date.accessioned2014-01-05T20:54:24Z
dc.date.available2014-01-05T20:54:24Z
dc.date.issued2004
dc.description.abstractThe Severe and Acute Respiratory Syndrome coronavirus (SARS CoV) is a newly-emerged virus that caused an outbreak of atypical pneumonia in the winter of 2002-2003. Polyclonal antibodies raised against the nucleocapsid (N) of the SARS CoV showed the localization of N to the cytoplasm and the nucleolus in virus-infected and N-expressing Vero E6 cells. Like other coronavirus N proteins, the SARS N is probably a phosphoprotein. N protein expressed in mammalian cells is apparently able to “spread” to neighboring cells. For N to spread to neighboring cells, it must be exported out of the expressing cells. This is shown by the immunoprecipitation of N from the culture medium of a stable cell line expressing myc-N. Deletion studies showed that the 27 kD C-terminal domain of N (C1/2) is the minimal region of N that can spread to other cells. The nucleolar localization and spreading of N are artefacts of fixation, reminiscent of other protein-transduction domain (PTD)-containing proteinsen_US
dc.description.accreditationWeb of Scienceen_US
dc.identifier.citationGoh, P., et al. (2004). Cellular Characterization of SARS Coronavirus Nucleocapsid. EXCLI Journal, 3: 91-104en_US
dc.identifier.issn1611-2156
dc.identifier.urihttp://hdl.handle.net/10566/913
dc.language.isoenen_US
dc.privacy.showsubmitterfalse
dc.publisherLeibniz Research Centre for Working Environment and Human Factorsen_US
dc.rights© 2004 Goh et al; licensee Leibniz Research Centre for Working Environment and Human Factors. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.status.ispeerreviewedtrue
dc.subjectSARSen_US
dc.subjectCoronavirusen_US
dc.subjectNucleocapsiden_US
dc.subjectNucleolusen_US
dc.subjectTransductionen_US
dc.subjectFixationen_US
dc.titleCellular Characterization of SARS Coronavirus Nucleocapsiden_US
dc.typeArticleen_US

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