Development of Y-STR genotyping systems suitable for sexual assault cases in South Africa

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dc.contributor.advisorBenjeddou, Mongi
dc.contributor.authorCloete, Kevin Wesley
dc.contributor.otherDept. of Biotechnology
dc.contributor.otherFaculty of Science
dc.date.accessioned2014-02-05T14:16:36Z
dc.date.accessioned2024-05-09T07:45:04Z
dc.date.available2010/05/25 21:51
dc.date.available2010/05/25
dc.date.available2014-02-05T14:16:36Z
dc.date.available2024-05-09T07:45:04Z
dc.date.issued2010
dc.descriptionMagister Scientiae - MScen_US
dc.description.abstractSexual assault is a significant problem facing the South African society. In this context, efficient but also affordable genotyping systems are needed for positive identification of criminals in incidences of sexual violence. The aim of this study was therefore to develop non-commercial Y-STR genotyping systems suitable for sexual assault cases in South Africa. Y-chromosome STR loci constituting the minimal haplotype are still the most widely used loci in investigating sexual assault cases despite the fact that DYS391 and DYS392 have shown low levels of polymorphism in Xhosa populations in Cape Town. The minimal haplotype was, therefore, further investigated in the Cape Muslim population. The Cape Muslim population generally exhibited high GD values among all the South African populations. These values were higher than 0.5 for most loci, and ranged from 0.447 for DYS391 to 0.957 for DYS385. The highest number of alleles in most loci was also recorded in this population. The overall assessment of the minimal haplotype has shown that this system is still a useful in investigating sexual assault case in many South African subpopulations. Therefore the exercise of internal validation of the minimal haplotype system was successfully carried out in the laboratory. The properties of additional novel and widely used STRs were also investigated in this study. Loci were successfully sequenced and allele nomenclature was assigned to them according to the ISFG guidelines.en_US
dc.description.countrySouth Africa
dc.identifier.urihttps://hdl.handle.net/10566/13278
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.rights.holderUniversity of the Western Capeen_US
dc.subjectForensicsen_US
dc.subjectShort tandem repeat (STR)en_US
dc.subjectY-locien_US
dc.subjectMinimal haplotype (MinHt)en_US
dc.subjectYHRDen_US
dc.subjectPolymerase chain reaction (PCR)en_US
dc.subjectElectrophoresisen_US
dc.subjectMultiplex PCRen_US
dc.subjectGenotypingen_US
dc.subjectGene diversityen_US
dc.subjectPolymorphismen_US
dc.titleDevelopment of Y-STR genotyping systems suitable for sexual assault cases in South Africaen_US
dc.typeThesisen_US

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