In vitro evaluation of the antiproliferative activity of Carpobrotus edulis on human neuroblastoma cells

dc.contributor.authorEkpo, Okobi Eko
dc.contributor.authorEnogieru, Adaze Bijou
dc.contributor.authorOmoruyi, Sylvester Ifeanyi
dc.date.accessioned2022-07-18T13:28:37Z
dc.date.available2022-07-18T13:28:37Z
dc.date.issued2021
dc.description.abstractNeuroblastoma is a solid neuroendocrine tumour located outside the cranial cavity and contributes about 15% of all cancer‑associated deaths in children. Treatment of neuroblastoma is quite challenging and involves the use of chemotherapy, surgery and radiotherapy. Despite treatment strategies, systemic toxicity are setbacks to patient well-being, hence the need for a new and affordable approach. Medicinal plants are of importance in the field of drug discovery for cancer as some notable anti-cancer agents have been isolated from them. In the present study, the anti-cancer activity of aqueous extract of Carpobrotus edulis (C. edulis), a ground-creeping edible medicinal plant was investigated in SK-N-BE(2) and SH-SY5Y neuroblastoma cells. The effect of C. edulis on cell viability and survival was determined using MTT (3-[4,5-dimethylthiazol-2-yl]􀀀 2,5 diphenyltetrazolium bromide) and clonogenic assays respectively. Apoptosis was determined using a Caspase-9 assay kit and flow cytometry was used to measure intracellular reactive oxygen species (ROS) and depolarization of mitochondrial membrane potential. The results show that C. edulis inhibits cell viability (IC50 of 0.86 mg/ml and 1.45 mg/ml for SK-N-BE (2) and SHSY5Y cells respectively) and colony formation in the neuroblastoma cells as well as induce apoptosis, which is evidenced by an increase in caspase-9 activity in the cells. C. edulis also led to a loss of mitochondrial membrane potential and increased production of ROS. Collectively, these results suggest that C. edulis induces cell death via induction of mitochondrial-mediated apoptosis and accumulation of intracellular ROS, thus providing a rationale for further investigations.en_US
dc.identifier.citationOmoruyi, S. I., Enogieru, A. B., & Ekpo, O. E. (2021). In vitro evaluation of the antiproliferative activity of Carpobrotus edulis on human neuroblastoma cells. Journal of Herbal Medicine, 30, 100519–. https://doi.org/10.1016/j.hermed.2021.100519en_US
dc.identifier.urihttps://doi.org/10.1016/j.hermed.2021.100519
dc.identifier.urihttp://hdl.handle.net/10566/7602
dc.language.isoenen_US
dc.publisherElsivieren_US
dc.subjectReactive oxygen speciesen_US
dc.subjectMitochondrial membrane potentialen_US
dc.subjectApoptosisen_US
dc.subjectNeuroblastomaen_US
dc.subjectCarpobrotus edulisen_US
dc.titleIn vitro evaluation of the antiproliferative activity of Carpobrotus edulis on human neuroblastoma cellsen_US
dc.typeArticleen_US

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