Purification and characterisation of plasmodium falciparum Hypoxanthine phosphoribosyltransferase

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dc.contributor.advisorMcIntosh, David B.
dc.contributor.advisorSewell, Trevor
dc.contributor.authorMurungi, Edwin Kimathi
dc.contributor.otherFaculty of Science
dc.date.accessioned2013-11-21T07:11:17Z
dc.date.accessioned2024-05-14T07:27:22Z
dc.date.available2009/11/03 12:56
dc.date.available2009/11/03
dc.date.available2013-11-21T07:11:17Z
dc.date.available2024-05-14T07:27:22Z
dc.date.issued2007
dc.descriptionMagister Scientiae - MScen_US
dc.description.abstractMalaria remains the most important parasitic disease worldwide. It is estimated that over 500 million infections and more that 2.7 million deaths arising from malaria occur each year. Most (90%) of the infections occur in Africa with the most affected groups being children of less than five years of age and women. this dire situation is exacerbated by the emrggence of drug resistant strains of Plasmodium falciparum. The work reported in this thesis focuses on improving the purification of PfHPRT by investigating the characteristics of anion exchange DE-52 chromatography (the first stage of purification), developing an HPLC gel filtration method for examining the quaternary structure of the protein and possible end stage purification, and initialcrystalization trials. a homology model of the open, unligaded PfHPRT is constructed using the atoomic structures of human, T.ccruz and STryphimurium HPRT as templates.en_US
dc.description.countrySouth Africa
dc.identifier.urihttps://hdl.handle.net/10566/14821
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.rights.holderUniversity of the Western Capeen_US
dc.subjectPlasmodium falciparumen_US
dc.subjectBioinformaticsen_US
dc.subjectProteinen_US
dc.subjectOligomeric structureen_US
dc.subjectHypoxanthine Phosphoribosyltransferaseen_US
dc.subjectCrystallizationen_US
dc.subjectHomology modelingen_US
dc.subjectPhotoaffinity labelingen_US
dc.titlePurification and characterisation of plasmodium falciparum Hypoxanthine phosphoribosyltransferaseen_US
dc.typeThesisen_US

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