Differential sensitivity of two endothelial cell lines to hydrogen peroxide toxicity: Relevance for in vitro studies of the blood–brain barrier

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dc.contributor.authorAlamu, Olufemi
dc.contributor.authorRado, Mariam
dc.contributor.authorEkpo, Okobi
dc.date.accessioned2022-05-30T09:34:48Z
dc.date.available2022-05-30T09:34:48Z
dc.date.issued2020
dc.description.abstractOxidative stress (OS) has been linked to blood–brain barrier (BBB) dysfunction which in turn has been implicated in the initiation and propagation of some neurological diseases. In this study, we profiled, for the first time, two endothelioma cell lines of mouse brain origin, commonly used as in vitro models of the blood–brain barrier, for their resistance against oxidative stress using viability measures and glutathione contents as markers. OS was induced by exposing cultured cells to varying concentrations of hydrogen peroxide and fluorescence microscopy/spectrometry was used to detect and estimate cellular glutathione contents. A colorimetric viability assay was used to determine changes in the viability of OS-exposed cells. Both the b.End5 and bEnd.3 cell lines investigated showed demonstrable content of glutathione with a statistically insignificant difference in glutathione quantity per unit cell, but with a statistically significant higher capacity for the b.End5 cell line for de novo glutathione synthesis. Furthermore, the b.End5 cells demonstrated greater oxidant buffering capacity to higher concentrations of hydrogen peroxide than the bEnd.3 cells. We concluded that mouse brain endothelial cells, derived from different types of cell lines, differ enormously in their antioxidant characteristics. We hereby recommend caution in making comparisons across BBB models utilizing distinctly different cell lines and require further prerequisites to ensure that in vitro BBB models involving these cell lines are reliable and reproducible.en_US
dc.identifier.citationAlamu, O. et al. (2020). Differential sensitivity of two endothelial cell lines to hydrogen peroxide toxicity: Relevance for in vitro studies of the blood–brain barrier. Cells, 9(2), 403. https://doi.org/10.3390/cells9020403en_US
dc.identifier.issn2073-4409
dc.identifier.urihttps://doi.org/10.3390/cells9020403
dc.identifier.urihttp://hdl.handle.net/10566/7474
dc.language.isoenen_US
dc.publisherMPDIen_US
dc.subjectOxidative stressen_US
dc.subjectBlood–brain barrieren_US
dc.subjectGlutathioneen_US
dc.subjectNeurological diseasesen_US
dc.titleDifferential sensitivity of two endothelial cell lines to hydrogen peroxide toxicity: Relevance for in vitro studies of the blood–brain barrieren_US
dc.typeArticleen_US

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