In vitro effects of aqueous extract of unfermented rooibos on human spermatozoa

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dc.contributor.authorHenkel, Ralf
dc.contributor.authorTakalani, Ndivhuho
dc.contributor.authorGbenga, Adefolaju
dc.contributor.authorChinyerum, Opuwari
dc.date.accessioned2022-05-16T11:59:18Z
dc.date.available2022-05-16T11:59:18Z
dc.date.issued2022
dc.description.abstractThe inability to conceive is a baleful experience for thousands of couples worldwide. Among different well-known reproductive techniques, medicinal plants have been utilized to treat male infertility. Medicinal plants, provide a therapeutic alternative, which is available and affordable for infertile couples. We investigated the direct effect of unfermented rooibos aqueous extract on human spermatozoa. Semen sam­ples (n = 50) collected from donors and patients consulting for fertility were reassigned as normal (n = 22) and abnormal (n = 28) samples based on the outcome of the baseline semen analysis, using the World Health Organization (WHO) cut off value. Semen samples were allowed to liquefy and subsequently washed with human tubular fluid in bovine serum albumin medium. The samples were then treated with aqueous extracts of unfermented rooibos (0, 0.15, 1.5, 15, 150 µg/ml) at 37° C for 1 h and assessed thereafter. Sperm motility, vitality, DNA fragmentation, intracellular reactive oxygen species and mitochondrial membrane potential in both groups remained unchanged (p > 0.05). However, aqueous extract of unfermented rooibos (only at 1.5 µg/ml) significantly increased capacitation and acrosome reaction in the abnormal sample group (p > 0.05). Unfermented rooibos aqueous extract had no del­eterious impact on human spermatozoa's function and might be attributed to its anti­oxidant properties.en_US
dc.identifier.citationTakalani, N., Adefolaju, G., Henkel, R. and Opuwari, C., 2022. In vitro effects of aqueous extract of unfermented rooibos on human spermatozoa. Andrologia,. DOI: http://doi.org/10.1111/and.14452en_US
dc.identifier.urihttp://doi.org/10.1111/and.14452
dc.identifier.urihttp://hdl.handle.net/10566/7399
dc.language.isoenen_US
dc.publisherWileyen_US
dc.subjectUnfermented rooibosen_US
dc.subjectRooibosen_US
dc.subjectSpermatozoaen_US
dc.subjectReactive oxygen speciesen_US
dc.subjectInfertilityen_US
dc.subjectAntioxidanten_US
dc.subjectMedicinal plantsen_US
dc.subjectReproductionen_US
dc.subjectHuman spermatozoaen_US
dc.titleIn vitro effects of aqueous extract of unfermented rooibos on human spermatozoaen_US
dc.typeArticleen_US

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