Characterisation of campylobacter concisus strains from South Africa using amplified fragment length polymorphism (AFLP) profiling and a genomospecies-specific polymerase chain reaction (PCR) assay: Identification of novel genomospecies and correlation with clinical data

dc.contributor.authorOn, S.L.W.
dc.contributor.authorSiemer, B. L.
dc.contributor.authorBrandt, S. M.
dc.contributor.authorChung, P.
dc.contributor.authorLastovica, Albert J.
dc.date.accessioned2016-06-08T12:16:59Z
dc.date.available2016-06-08T12:16:59Z
dc.date.issued2013
dc.description.abstractAmplified Fragment Length Polymorphism (AFLP) profiling was used to evaluate the distribution of phenotypically indistinguishable, but genetically distinct, among Campylobacter concisus strains from South Africa. A Polymerase Chain Reaction (PCR) assay described for identifying strains belonging to Genomospecies 1 and 2 was applied in this study. Forty-seven C. concisus strains were studied in total, of which 42 were of South African origin. Forty of the South African isolates were assigned to the major existing genomospecies typified by the type strain of oral origin (GS1), and reference strains from bloody diarrhoea (GS2). Eighteen South African isolates were distributed in the GS1 cluster including two oral strains. Twenty-two faecal South African isolates clustered with reference GS2 strains. Two novel genomospecies (GS 5 and 6) were inferred by their AFLP profile characteristics. Use of an existing PCR assay first described for identification of GS1 and GS2 strains generally indicated that the tool was accurate, although the novel genomospecies described here yielded an amplicon in the GS2 assay. No consistent clinical pattern among the diarrhoea South African strains could be discerned. The study extends the known genetic diversity among C. concisus, elucidates the presence of multiple genomospecies in South Africa, and confirms for the first time an association of GS1 with diarrhoea as well as the utility (with caveats) of a PCR assay for identifying GS1 and GS2 strains.en_US
dc.identifier.citationOn, S.L.W. et al. (2013). Characterisation of campylobacter concisus strains from South Africa using amplified fragment length polymorphism (AFLP) profiling and a genomospecies-specific polymerase chain reaction (PCR) assay: Identification of novel genomospecies and correlation with clinical data. African Journal of Microbiology Research, 7(18): 1845 - 1851en_US
dc.identifier.issn1991-637X
dc.identifier.urihttp://hdl.handle.net/10566/2293
dc.language.isoenen_US
dc.privacy.showsubmitterFALSE
dc.publisherAcademic Journalsen_US
dc.rights.uriAcademic Journals strongly supports the Open Access initiative. Abstracts and full texts of all articles published by Academic Journals are freely accessible to everyone immediately after publication.
dc.source.urihttp://dx.doi.org/10.5897/AJMR12.2182
dc.status.ispeerreviewedTRUE
dc.subjectCampylobacteren_US
dc.subjectPeriodontal diseaseen_US
dc.subjectGastroenteritisen_US
dc.subjectDiarrhoeaen_US
dc.subjectRed Cross Children's hospitalen_US
dc.subjectThe Cape Town Protocolen_US
dc.subjectBacteriaen_US
dc.subjectAmplified Fragment Length Polymorphism (AFLP)en_US
dc.subjectPolymerase Chain Reaction (PCR)en_US
dc.subjectPaediatric medicineen_US
dc.titleCharacterisation of campylobacter concisus strains from South Africa using amplified fragment length polymorphism (AFLP) profiling and a genomospecies-specific polymerase chain reaction (PCR) assay: Identification of novel genomospecies and correlation with clinical dataen_US
dc.typeArticleen_US

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