Efficient molecular cloning of environmental DNA from geothermal sediments
| dc.contributor.author | Wilkinson, Dianne E. | |
| dc.contributor.author | Jaenicke, Thomas | |
| dc.contributor.author | Cowan, Donald A. | |
| dc.date.accessioned | 2010-10-21T09:17:49Z | |
| dc.date.available | 2010-10-21T09:17:49Z | |
| dc.date.issued | 2002 | |
| dc.description.abstract | An efficient and simple method for constructing an environmental library using mechanically sheared DNA obtained directly from geothermal sediments is presented. The method is based on blunt-end modification of DNA fragments followed by 3' -adenylation using Vent DNA polymerase and Taq DNA polymerase, respectively. The prepared DNA fragments are then ligated into a TA cloning vector and used in the transformation of Escherichia coli. This method has been successfully applied to the cloning of ORFs derived from uncultivated prokaryotes present in geothermal sediment. | en_US |
| dc.identifier.citation | Wilkinson, D., Jaenicke, T., Cowan, D.A. (2002). Efficient cloning of DNA from environmental sources. Biotechnology Letters, 24(2):155-161. | en_US |
| dc.identifier.uri | http://hdl.handle.net/10566/161 | |
| dc.inquiries | dcowan@uwc.ac.za | |
| dc.language.iso | en | en_US |
| dc.privacy.showsubmitter | true | |
| dc.publisher | Kluwer Academic Publishers | en_US |
| dc.rights | This is the author post-print of an article published by Kluwer Academic Publishers. This version may be freely used in non-commercial applications, subject to full acknowledgement. | |
| dc.source.uri | http://dx.doi.org/10.1023/A:1013825600643 | |
| dc.status.ispeerreviewed | true | |
| dc.subject | Culture-independent cloning | en_US |
| dc.subject | Environmental DNA library | en_US |
| dc.subject | Geothermal sediment | en_US |
| dc.subject | Metagenome | en_US |
| dc.title | Efficient molecular cloning of environmental DNA from geothermal sediments | en_US |
| dc.type | Article | en_US |