Differential effects of normoxic versus hypoxic derived breast cancer paracrine factors on brain endothelial cells

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dc.contributor.authorRado, Mariam
dc.contributor.authorFlepisi, Brian
dc.contributor.authorFisher, David
dc.date.accessioned2022-01-17T11:06:00Z
dc.date.available2022-01-17T11:06:00Z
dc.date.issued2021
dc.description.abstractThe blood-brain barrier (BBB) is a central nervous system protective barrier formed primarily of endothelial cells that regulate the entry of substances and cells from entering the brain. However, the BBB integrity is disrupted in disease, including cancer, allowing toxic substances, molecules, and circulating cells to enter the brain. This study aimed to determine the mitochondrial changes in brain endothelial cells co-cultured with cancer cells. Method: Brain endothelial cells (bEnd.3) were co-cultivated with various concentrations of breast cancer (MCF7) conditioned media (CM) generated under normoxic (21% O2 ) and hypoxic conditions (5% O2 ). The mitochondrial activities (including; dehydrogenases activity, mitochondrial membrane potential (∆Ψm), and ATP generation) were measured using Polarstar Omega B.M.G-Plate reader. Trans-endothelial electrical resistance (TEER) was evaluated using the EVOM system, followed by quantifying gene expression of the endothelial tight junction (ETJs) using qPCR. Results: bEnd.3 cells had reduced cell viability after 72 h and 96 h exposure to MCF7CM under hypoxic and normoxic conditions.en_US
dc.identifier.citationRado, M. et al. (2021). Differential effects of normoxic versus hypoxic derived breast cancer paracrine factors on brain endothelial cells. Biology, 10(12), 1238. 10.3390/biology10121238en_US
dc.identifier.issn2079-7737
dc.identifier.uri10.3390/biology10121238
dc.identifier.urihttp://hdl.handle.net/10566/7107
dc.language.isoenen_US
dc.publisherMPDIen_US
dc.subjectBreast canceren_US
dc.subjectCancer secretomeen_US
dc.subjectBrain endothelial cellsen_US
dc.subjectBlood-brain barrieren_US
dc.titleDifferential effects of normoxic versus hypoxic derived breast cancer paracrine factors on brain endothelial cellsen_US
dc.typeArticleen_US

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