Physiological and biochemical characterization, of antimony stress, responses in Phaseolus vulgaris

dc.contributor.advisorKeyster, Marshall
dc.contributor.authorNiekerk, Lee-Ann Tina
dc.date.accessioned2018-08-13T08:34:12Z
dc.date.accessioned2024-05-09T07:47:02Z
dc.date.available2020-08-31T22:10:05Z
dc.date.available2024-05-09T07:47:02Z
dc.date.issued2018
dc.descriptionMagister Scientiae - MSc (Biotechnology)
dc.description.abstractThe mining industry in South Africa is of immense importance as this sector contributes largely to the countries income. In the Limpopo province, a large production of antimony (Sb) is generated per year. Antimony (Sb) is a trace element, which accumulates in the environment through anthropogenic activities, such as mining and smelting industries. Antimony is toxic to all living organisms and plants, and it is found to increase the peroxidation of membrane lipids and encourage an antioxidant response. Sb contamination in plants also accounts for DNA damage. The reduction in yield is due to the disruption of plant metabolism by reactive oxygen species (ROS). To combat abiotic stresses, plants have generated a signalling network that utilises multiple growth regulators that would offer protection against the stress. An increase in ROS is one of the responses to abiotic stresses. ROS is generated in response to the pants interaction with heavy metals, through the Harber-Weiss reaction. ROS compounds include: superoxide, hydrogen peroxide and hydroxyl radicals. Under normal conditions ROS molecules are produced as by-products, however, under stressful conditions the production of ROS molecules are increased to levels where they are detrimental to the plants. Therefore, the accumulation of ROS results in damage to proteins, lipids, carbohydrates and DNA which would lead to cellular death. ROS accumulation is thought to be a result of the disruption in the balance of ROS production and the anti-oxidation systems. The antioxidative system is thus introduced to restore the balance of ROS molecule production and to combat oxidative damage caused by the ROS molecules. The anti-oxidative system consists of various enzymes: superoxide dismutase, catalase, and ascorbate peroxidase and glutathione reductase. Each antioxidant scavenges one or two ROS molecules.
dc.identifier.urihttps://hdl.handle.net/10566/13459
dc.language.isoen
dc.publisherUniversity of the Western Cape
dc.rights.holderUniversity of the Western Cape
dc.subjectAscorbate peroxidase, Catalase, Contender, Deoxyribonucleic acid, Ethylenediaminetetraacetic acid
dc.titlePhysiological and biochemical characterization, of antimony stress, responses in Phaseolus vulgaris

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
2293-2538-1-RV.pdf
Size:
4.15 MB
Format:
Adobe Portable Document Format