Comparative analysis of testis and ovary transcriptomes in zebrafish by combining experimental and computational tools

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dc.contributor.authorLi, Yang
dc.contributor.authorChia, Jer, M
dc.contributor.authorBartfai, Richard
dc.contributor.authorChristoffels, Alan
dc.contributor.authorYue, Gen, H
dc.contributor.authorDing, Ke
dc.contributor.authorHo, Mei, Y
dc.contributor.authorHill, James, A
dc.contributor.authorStupka, Elia
dc.contributor.authorOrban, Laszlo
dc.date.accessioned2015-09-10T09:26:40Z
dc.date.available2015-09-10T09:26:40Z
dc.date.issued2004
dc.description.abstractStudies on the zebrafish model have contributed to our understanding of several important developmental processes, especially those that can be easily studied in the embryo. However, knowledge on late events such as gonad differentiation in the zebrafish is still limited. Here an analysis on the gene sets is expressed in the adult zebrafish testis and ovary in an attempt to identify genes with potential role in (zebra)fish gonad development and function. We produced 10 533 expressed sequence tags (ESTs) from zebrafish testis or ovary and downloaded an additional 23 642 gonad-derived sequences from the zebrafish EST database. We clustered these sequences together with over 13 000 kidney-derived zebrafish ESTs to study partial transcriptomes for these three organs. We searched for genes with gonad-specific expression by screening macroarrays containing at least 2600 unique cDNA inserts with testis-, ovary- and kidney-derived cDNA probes. Clones hybridizing to only one of the two gonad probes were selected, and subsequently screened with computational tools to identify 72 genes with potentially testis-specific and 97 genes with potentially ovary-specific expression, respectively. PCR-amplification confirmed gonad-specificity for 21 of the 45 clones tested (all without known function). Our study, which involves over 47 000 EST sequences and specialized cDNA arrays, is the first analysis of adult organ transcriptomes of zebrafish at such a scale. The study of genes expressed in adult zebrafish testis and ovary will provide useful information on regulation of gene expression in teleost gonads and might also contribute to our understanding of the development and differentiation of reproductive organs in vertebrates.en_US
dc.description.sponsorshipResearch grants from Temasek Lifesciences Laboratory Agency for Science, Technology and Researchen_US
dc.identifier.citationLi, Yang., et.al. (2004). Comparative analysis of testis and ovary transcriptomes in zebrafish by combining experimental and computational tools. Comparative and Functional Genomics. 5: 403–418en_US
dc.identifier.urihttp://hdl.handle.net/10566/1593
dc.language.isoenen_US
dc.privacy.showsubmitterfalse
dc.publisherWileyen_US
dc.rightsCopyright © 2004 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.source.urihttp://dx.doi.org/10.1002/cfg.418
dc.status.ispeerreviewedtrue
dc.subjectGonad differentiationen_US
dc.subjectZebrafishen_US
dc.subjectReproductionen_US
dc.subjectTeleost gonadsen_US
dc.subjectReproductive organsen_US
dc.subjectVertabratesen_US
dc.titleComparative analysis of testis and ovary transcriptomes in zebrafish by combining experimental and computational toolsen_US
dc.typeArticleen_US

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