Monoclonal Antibodies targeting the HR2 Domain and the region immediately upstream of the HR2 of the S Protein neutralize in Vitro Infection of Severe Acute Respiratory Syndrome Coronavirus

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dc.contributor.authorLip, Kuo-Ming
dc.contributor.authorShen, Shuo
dc.contributor.authorYang, Xiaoming
dc.contributor.authorKeng, Choong-Tat
dc.contributor.authorZhang, Aihua
dc.contributor.authorOh, Hsueh-Ling Janice
dc.contributor.authorLi, Zhi-Hong
dc.contributor.authorHwang, Le-Ann
dc.contributor.authorChou, Chih-Fong
dc.contributor.authorFielding, Burtram C.
dc.contributor.authorTan, Timothy H.P.
dc.contributor.authorMayrhofer, Josef
dc.contributor.authorFalkner, Falko G.
dc.contributor.authorFu, Jianlin
dc.contributor.authorLim, Seng Gee
dc.contributor.authorHong, Wanjin
dc.contributor.authorTan, Yee-Joo
dc.date.accessioned2014-01-06T20:24:37Z
dc.date.available2014-01-06T20:24:37Z
dc.date.issued2006
dc.description.abstractWe have previously shown that an Escherichia coli-expressed, denatured spike (S) protein fragment of the severe acute respiratory coronavirus, containing residues 1029 to 1192 which include the heptad repeat 2 (HR2) domain, was able to induce neutralizing polyclonal antibodies (C. T. Keng, A. Zhang, S. Shen, K. M. Lip, B. C. Fielding, T. H. Tan, C. F. Chou, C. B. Loh, S. Wang, J. Fu, X. Yang, S. G. Lim, W. Hong, and Y. J. Tan, J. Virol. 79:3289–3296, 2005). In this study, monoclonal antibodies (MAbs) were raised against this fragment to identify the linear neutralizing epitopes in the functional domain and to investigate the mechanisms involved in neutralization. Eighteen hybridomas secreting the S protein-specific MAbs were obtained. Binding sites of these MAbs were mapped to four linear epitopes. Two of them were located within the HR2 region and two immediately upstream of the HR2 domain. MAbs targeting these epitopes showed in vitro neutralizing activities and were able to inhibit cell-cell membrane fusion. These results provide evidence of novel neutralizing epitopes that are located in the HR2 domain and the spacer region immediately upstream of the HR2 of the S protein.en_US
dc.description.accreditationWeb of Scienceen_US
dc.identifier.citationLip, K., et al. (2006). Monoclonal Antibodies targeting the HR2 Domain and the region immediately upstream of the HR2 of the S Protein neutralize in Vitro Infection of Severe Acute Respiratory Syndrome Coronavirus, Journal of Virology, 80(2): 941-950en_US
dc.identifier.issn0022-538X
dc.identifier.urihttp://hdl.handle.net/10566/915
dc.language.isoenen_US
dc.privacy.showsubmitterfalse
dc.publisherAmerican Society for Microbiologyen_US
dc.rightsCopyright American Society for Microbiology. This file may be freely used for educational purposes, as long as it is not altered in any way. Acknowledgement of the authors and the source is required.
dc.source.urihttp://dx.doi.org/10.1128/JVI.80.2.941-950.2006
dc.status.ispeerreviewedtrue
dc.subjectInfluenza virusen_US
dc.subjectSevere acute respiratory syndrome coronavirus (SARS-CoV)en_US
dc.titleMonoclonal Antibodies targeting the HR2 Domain and the region immediately upstream of the HR2 of the S Protein neutralize in Vitro Infection of Severe Acute Respiratory Syndrome Coronavirusen_US
dc.typeArticleen_US

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