Characterization of a unique group-specific protein (U122) of the severe acute respiratory syndrome coronavirus

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dc.contributor.authorFielding, Burtram C.
dc.contributor.authorTan, Yee-Joo
dc.contributor.authorShen, Shuo
dc.contributor.authorTan, Timothy H.P.
dc.contributor.authorOoi, Eng-Eong
dc.contributor.authorLim, Seng Gee
dc.contributor.authorHong, Wanjin
dc.contributor.authorGoh, Phuay-Yee
dc.date.accessioned2013-10-12T04:36:03Z
dc.date.available2013-10-12T04:36:03Z
dc.date.issued2004
dc.description.abstractA novel coronavirus (CoV) has been identified as the etiological agent of severe acute respiratory syndrome (SARS). The SARS-CoV genome encodes the characteristic essential CoV replication and structural proteins. Additionally, the genome contains six group-specific open reading frames (ORFs) larger than 50 amino acids, with no known homologues. As with the group-specific genes of the other CoVs, little is known about the SARS-CoV group-specific genes. SARS-CoV ORF7a encodes a putative unique 122-amino-acid protein, designated U122 in this study. The deduced sequence contains a probable cleaved signal sequence and a C-terminal transmembrane helix, indicating that U122 is likely to be a type I membrane protein. The C-terminal tail also contains a typical endoplasmic reticulum (ER) retrieval motif, KRKTE. U122 was expressed in SARS-CoVinfected Vero E6 cells, as it could be detected by Western blot and immunofluorescence analyses. U122 is localized to the perinuclear region of both SARS-CoV-infected and transfected cells and colocalized with ER and intermediate compartment markers. Mutational analyses showed that both the signal peptide sequence and ER retrieval motif were functional.en_US
dc.description.accreditationWeb of Scienceen_US
dc.identifier.citationFielding, B., et al. (2004). Characterization of a unique group-specific protein (U122) of the severe acute respiratory syndrome coronavirus. Journal of Virology, 78(14): 7311-7318en_US
dc.identifier.issn0022-538X
dc.identifier.urihttp://hdl.handle.net/10566/741
dc.language.isoenen_US
dc.privacy.showsubmitterfalse
dc.publisherAmerican Society for Microbiologyen_US
dc.rightsCopyright American Society for Microbiology. This file may be freely used for educational purposes, as long as it is not altered in any way. Acknowledgement of the authors and the source is required.
dc.source.urihttp://dx.doi.org/10.1128/JVI.78.14.7311-7318.2004
dc.status.ispeerreviewedtrue
dc.subjectSevere acute respiratory syndrome (SARS)en_US
dc.subjectNovel coronavirus (CoV)en_US
dc.titleCharacterization of a unique group-specific protein (U122) of the severe acute respiratory syndrome coronavirusen_US
dc.typeArticleen_US

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