Browsing by Author "Siebrits, Michaela Nicole"

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    To explore the effect of prednisolone on blood-brain barrier endothelial cells treated with lipopolysaccharides.
    (Universty of the Western Cape, 2024) Siebrits, Michaela Nicole; Makhathini, Khayelihle Brain
    The blood-brain barrier (BBB) is primarily regulated by highly selective endothelial cells. Inflammation of the brain capillaries can compromise the BBB’s functioning. Methylprednisolone (M-Pred), a synthetic glucocorticoid, is generally prescribed to treat inflammation, though little is known of its effect on inflamed brain capillaries. This study evaluated the effectiveness of M-Pred in ameliorating the LPS-induced inflammatory response in the in vitro BBB model. Cultured brain endothelial cells (bEnd.3) were exposed to LPS (500, 1000, 2000 ng/ml) and co-treated with selected concentrations of M-Pred (10, 20, 40 μM). Cell viability, cell toxicity, and cell proliferation, bEnd.3 monolayer permeability (Transendothelial Electrical Resistance) were investigated, while ELISA kits were used to quantify pro-inflammatory cytokines, TNF-α and IL-1β.