Browsing by Author "Olowu, Rasaq A."
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Item Correction: Baker, P. et al. electrochemical aptasensor for endocrine disrupting 17β-estradiol based on a poly(3,4-ethylenedioxylthiopene)-gold nanocomposite platform. Sensors 2010, 10, 9872-9890(MDPI, 2011) Olowu, Rasaq A.; Arotiba, Omotayo A.; Mailu, Stephen N.; Waryo, Tesfaye T.; Baker, Priscilla; Iwuoha, Emmanuel I.Herewith please find corrected structures for Figure 8 in our paper published in Sensors in 2010.Item Electrochemical aptasensor for endocrine disrupting 17β-estradiol based on a poly(3,4-ethylenedioxylthiopene)-gold nanocomposite platform(MDPI, 2010) Olowu, Rasaq A.; Mailu, Stephen N.; Waryo, Tesfaye T.; Baker, Priscilla; Iwuoha, Emmanuel I.; Arotiba, Omotayo A.A simple and highly sensitive electrochemical DNA aptasensor with high affinity for endocrine disrupting 17β-estradiol, was developed. Poly(3,4-ethylenedioxylthiophene) (PEDOT) doped with gold nanoparticles (AuNPs) was electrochemically synthesized and employed for the immobilization of biotinylated aptamer towards the detection of the target. The diffusion coefficient of the nanocomposite was 6.50 × 10−7 cm2 s−1, which showed that the nanocomposite was highly conducting. Electrochemical impedance investigation also revealed the catalytic properties of the nanocomposite with an exchange current value of 2.16 × 10−4 A, compared to 2.14 × 10−5 A obtained for the bare electrode. Streptavidin was covalently attached to the platform using carbodiimide chemistry and the aptamer immobilized via streptavidin—biotin interaction. The electrochemical signal generated from the aptamer–target molecule interaction was monitored electrochemically using cyclic voltammetry and square wave voltammetry in the presence of [Fe(CN)6]−3/−4 as a redox probe. The signal observed shows a current decrease due to interference of the bound 17β-estradiol. The current drop was proportional to the concentration of 17β-estradiol. The PEDOT/AuNP platform exhibited high electroactivity, with increased peak current. The platform was found suitable for the immobilization of the DNAaptamer. The aptasensor was able to distinguish 17β-estradiol from structurally similar endocrine disrupting chemicals denoting its specificity to 17β-estradiol. The detectable concentration range of the 17β-estradiol was 0.1 nM–100 nM, with a detection limit of 0.02 nM.Item Impact of climate change and anthropogenic activities on renewable coastal resources and biodiversity in Nigeria(Academic Journals, 2012) Amosu, Albert O.; Bashorun, O.W.; Babalola, O.O.; Olowu, Rasaq A.; Togunde, K.A.Climates related disaster is greatly human induced warming, leading to substantial fluctuations in earth temperature which is currently a global issue of environmental concern. Human proximity and pressure in and around the coastal region has threatened flora, fauna and micro-organic resources of economic importance in most developing nations. Two-third of the word populations lives in or around the coast and 16 of the 23 world mega cities are currently in the world coastal belt. Transitively, the coastal environment, which harbors the highest percentage of biodiversity, is significantly important for economic activities and leisure. Nigeria's climate may witness increases in temperature, rainfall, storms, and sea levels throughout the twenty-first century and improper management of these desiccations can result into degradation in some parts of the country. Apart from climate change, coastal environment is subject to various anthropogenic impacts, often associated with high population, industrial and agricultural activities. Both disasters have led to heat stress, sea level rise, and erosion, salinization of the soil, evapo- transpiration, desertification and others. The overall resultant effects are feasible on environmental indicators including renewable coastal resources. It is therefore recommended among others that thinking globally and acting locally on environmental issues could save our coastal nation.