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Browsing by Author "Nembaware, Victoria P."

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    Analyses of sequence divergence using completely sequenced genomes
    (University of the Western Cape, 2003) Nembaware, Victoria P.; Seoighe, Cathal
    Using the complete genome, Saccharomyces cerevisiae, which duplicated after its speciation fuom Kluyveromyces lactics, a dataset of 119 putative S. cerevisiae - K. lactis ortholog-pairs was constructed. S. cerevisiae paralogous pairs that are likely to have duplicated during the whole genome duplication of S. cerevisiae were obtained and the approach taken in our previous work (Nembaware et al., 20OZ), was repeated to test whether the presence of a paralogue in S. cerevisiae had an effect on the rate of sequence divergence of the 119 pairs of orthologous genes. We found, however, that substitutions at synonymous sites had reached saturation and this prevented us from being able to repeat the previous finding with S. cerevistae and K. lactis . From this study a publicly available web-server (http://hamlyn.sanbi.ac.zal-victoria) that automates the calculation of Ka:Ks values given a pairs homologous CDS sequences is presented.

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