Browsing by Author "Ndiko, Ludidi"

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    Antioxidant responses are associated with differences in drought tolerance between maize and sorghum
    (Arid land institute, 2021) Elbasheir, Ali Ali Elnaeim; Ndiko, Ludidi
    Drought is a major cause of decreased yield in crops worldwide. Sorghum (Sorghum bicolor) and maize (Zea mays) are two of the key crops in Africa serving as human food as well as livestock feed. For improved crop production, selection for drought resilient genotypes is imperative and the biological basis for drought tolerance ought to be fully understood to achieve such selection. Sorghum can tolerate drought better than maize and it is a key model for studying the physiological and biochemical mechanisms conferring drought tolerance. In this study, comparative analyses in terms of changes in growth, chlorophyll content, ROS content, lipid peroxidation level and the activity of antioxidant enzymes were investigated. Exposure to drought triggered ROS generation in both plant species. However, sorghum showed less cell damage under water deficit compared to maize. Furthermore, differences in antioxidant enzyme activity between maize and sorghum were identified. Our findings reveal differences in and association between the physiological and biochemical responses of maize and sorghum to drought, which may be relevant for breeding drought tolerant crops.
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    Genome-wide characterization of DREB transcription factors in Medicago truncatula: Insights into their roles in development and abiotic stress response
    (Elsevier Inc, 2025) Ndiko, Ludidi; Haddoudi, Loua; Ayadi, Mariem
    Dehydration-responsive-element binding (DREB) proteins play a crucial role in drought, salt, and environmental stress tolerance. In this study, we identified and annotated fifty-four DREB genes from the Medicago truncatula genome. These genes were analyzed at the molecular level, focusing on gene classification, genomic organization, phylogeny, synteny, structural features, and expression profiles. Phylogenetic analysis revealed that MtDREB proteins are categorized into six subgroups (A1–A6), with highly conserved motif compositions among them. Expression profiling showed that MtDREB genes are differentially expressed in various plant organs and under abiotic stresses (cold, salinity, and dehydration), with 30 % exhibiting high expression during flowering and development. Data from RNA-seq and microarrays demonstrated that 76 % of MtDREB genes are differentially expressed under at least one stress condition, indicating their involvement in various signaling pathways activated by abiotic stresses. Notably, MtDREB05, primarily induced under osmotic stress, appears to be a promising candidate for improving abiotic stress tolerance. These findings will enhance our understanding of the DREB family and aid in functional validation of DREBgenes in M. truncatula and related forage species.
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    Indigenous African cereal crops can contribute to mitigation of the impact of climate change on food security
    (Arid land institute, 2021) Gerhard, Basson; Elbasheir, Ali Ali Elnaeim; Ndiko, Ludidi
    Zea mays L. (maize) is one of the top three cultivated cereals globally, along with wheat and rice. The United States, China, and Brazil are among the largest producers of maize, producing approximately 79% of the world’s maize. Maize is used to produce human food and animal feed. It is also used to produce industrial products such as starch sweeteners, oil, beverages and bioethanol. South Africa produces maize as well. However, this production is relatively insignificant compared to the major producers. Furthermore, South Africa is a semi-arid country and as such receives less rainfall and has annual droughts. This has negative implications on maize production, which threatens food security. The sole reliance on a limited number of staple cereals is not a sustainable option. In order to truly improve food security, the diversification of staple cereals is necessary. Therefore, this review aims at discussing the diversification of staple cereals in southern Africa, specifically focusing on sorghum, pearl millet, finger millet and teff. These African indigenous cereals are known for their environmental resilience as well as having nutritional benefits. Southern Africa will experience more droughts in the future as a result of climate change, which will undoubtedly impact maize yields. Therefore, it is important that efforts are intensified to ensure that indigenous drought-adapted crops are fully exploited to improve future food security.