Browsing by Author "Li, Yang"

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    Comparative analysis of testis and ovary transcriptomes in zebrafish by combining experimental and computational tools
    (Wiley, 2004) Li, Yang; Chia, Jer, M; Bartfai, Richard; Christoffels, Alan; Yue, Gen, H; Ding, Ke; Ho, Mei, Y; Hill, James, A; Stupka, Elia; Orban, Laszlo
    Studies on the zebrafish model have contributed to our understanding of several important developmental processes, especially those that can be easily studied in the embryo. However, knowledge on late events such as gonad differentiation in the zebrafish is still limited. Here an analysis on the gene sets is expressed in the adult zebrafish testis and ovary in an attempt to identify genes with potential role in (zebra)fish gonad development and function. We produced 10 533 expressed sequence tags (ESTs) from zebrafish testis or ovary and downloaded an additional 23 642 gonad-derived sequences from the zebrafish EST database. We clustered these sequences together with over 13 000 kidney-derived zebrafish ESTs to study partial transcriptomes for these three organs. We searched for genes with gonad-specific expression by screening macroarrays containing at least 2600 unique cDNA inserts with testis-, ovary- and kidney-derived cDNA probes. Clones hybridizing to only one of the two gonad probes were selected, and subsequently screened with computational tools to identify 72 genes with potentially testis-specific and 97 genes with potentially ovary-specific expression, respectively. PCR-amplification confirmed gonad-specificity for 21 of the 45 clones tested (all without known function). Our study, which involves over 47 000 EST sequences and specialized cDNA arrays, is the first analysis of adult organ transcriptomes of zebrafish at such a scale. The study of genes expressed in adult zebrafish testis and ovary will provide useful information on regulation of gene expression in teleost gonads and might also contribute to our understanding of the development and differentiation of reproductive organs in vertebrates.
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    Models and applications of wireless networks in rural environments
    (University of the Western Cape, 2005) Li, Yang; Agbinya, Johnson I.; Dept. of Computer Science; Faculty of Science
    With the unprecedented growth of the communication industry that the world is experiencing, the demand from rural inhabitants for high quality communications at an economically affordable cost is growing. However, rural areas are rather restricted from deploying communication services due to the rough natural environment, and the shortage of rudimentary communication facilities and technical personnel. Appropriate models for building rural wireless networks and a concomitant simulation environment are, therefore, expected to enable the construction of technologically-optimal and economically-efficient networks in specified rural areas.The research has set up two independent models, one for the economic need and the other for the technical need of building networks in rural areas. One model was the Impact of Telecommunications Model, which disclosed the importance of building a wireless network in specified rural areas by choosing an economic parameter to forecast the profitability of the network. The other was the Service Model, which collected primitive data from given rural areas and abstracted these data by flowing them through four technical layers to form the predicted technical wireless network. Both of the models had been applied to real-world cases to demonstrate how to use them.A simulation environment was finally designed and implemented to realize the above two models for the sake of instantiation. This environment could simulate the specified rural network by constructing a wireless network on the invented areas and evaluating its quality and economic efficiency. It was written in Scilab simulation language, which was an open source.