Browsing by Author "Hüsselmann, Lizex"

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Application of nanotechnology and proteomic tools in crop development towards sustainable agriculture
    (Springer, 2024) Daniel, Augustine Innalegwu; Hüsselmann, Lizex; Shittu, Oluwatosin Kudirat; Gokul, Arun
    The increase in global population which translates to increased demand for food called for urgent attention from key players and policy makers in agricultural sector. Also, the effects of climate change and its consequent biotic and abiotic stresses in plants has greatly affect the sustainability of agriculture and production of food. These challenges require novel and sustainable approaches to improve the quality and yield of crops. The vast application of nanoparticles in different fields of study is attributed to their distinct chemical and physical characteristics. However, in agriculture, their application is limited because of their safety concern. Currently, research is tailored to study the response of plants to various nanoparticle treatments; however, these studies are inconclusive due to limited knowledge on the mechanisms of plant–nanoparticle interactions. Recently, studies on nanobiotechnology have taken a new dimension from preliminary bioassay experiments to more complex, research-oriented studies using various omics tools. Changes in protein expression caused by plant–nanoparticle interaction at any developmental stages, or tissue types may be investigated using suitable proteomics techniques. This review discussed the various applications of nanotechnology and proteomic tools in plant growth and development. Plant–nanoparticle interactions and the application of nanoparticles and proteomic tools in genetic engineering of plants to attain agricultural sustainability and food safety are also discussed. Informative and thorough understanding of plant–nanoparticle interaction will serve as a blueprint that will enable plant scientist and genetic engineers to develop plant biomarkers and explore their potential application for crop improvement.
  • Thumbnail Image
    Item
    Comparative whole corona fingerprinting and protein adsorption thermodynamics of PLGA and PCL nanoparticles in human serum
    (Elsevier, 2020) Ndumiso, Myolisi; Buchtová, Nela; Hüsselmann, Lizex
    Nanoparticles (NPs) based on biocompatible and biodegradable polymers such as poly(lactic-co-glycolic acid) (PLGA) and polycaprolactone (PCL) represent effective systems for systemic drug delivery. Upon injection into the blood circuit, the NP surface is rapidly modified due to adsorption of proteins that form a ‘protein corona’ (PC). The PC plays an important role in cellular targeting, uptake and NP bio-distribution. Hence, the study of interactions between NPs and serum proteins appears as key for biomedical applications and safety of NPs. In the present work, we report on the comparative protein fluorescence quenching extent, thermodynamics of protein binding and identification of proteins in the soft and hard corona layers of PLGA and PCL NPs. NPs were prepared via a single emulsion-solvent evaporation technique and characterized with respect to size, zeta potential, surface morphology and hydrophobicity. Protein fluorescence quenching experiments were performed against human serum albumin. The thermodynamics of serum protein binding onto the NPs was studied using isothermal titration calorimetry. Semi-quantitative analysis of proteins in the PC layers was conducted using gel electrophoresis and mass spectrometry using human serum.