Browsing by Author "Govender Liezel"

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    The apoptotic effects of Artemisia afra phytotherapy and modulation of an immune biomarker in cells
    (University of the Western Cape, 2024) Govender Liezel; Johnson Quinton
    Aim: Artemisia afro phytotherapy was assessed for its apoptotic effects on HeLa, Caski and CHO22 cells especially, and to determine its immune modulating effects on whole blood cell s. Apoptosis methods: For its apoptotic evaluation, aqueous extracts of phytotherapy (4, 6. 8 and 10 mg/ml) were incubated with the various ce ll lines over time (24 hours). L ight microscopy and flow cytometric analysis were completed to assess the possible pro--apoptotic activity of the extracts. The following assays were used as detectors for speci tic apoptotic markers: The ApoPercentageTM Apoptosis assay is a colorimetric assay system that was used to detect and quantify apoptosis. The Annexin V assay was used Lo detect phosphaticly l serine. The Caspase-3 assay was used to detect cells undergoing apoptosis through activation of the caspase-3 pathway. An induction of mitochondrial depolari zation in ce lls undergoing apoptosis was detected using the TMRE assay. Apoptosis results: Ir was noted in the A nnexin V assay that an increase in apoptotic ce lls occurred, as measured by the increased amounts of Annex in V V-PF: and dye 7 amino actinomycin (7aacl) in all three cell lines. An increase in the presence of the dye in cells implied that the membrane integrity of the ce ll lines was breached. \vhich suggests that these cells have undergone the process of apoptosis. Furthermore. an increase in the dose of the phytotherapy. led to a decrease in membrane integrity of these ce ll lines.