Browsing by Author "Daniel, Augustine Innalegwu"

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    Application of nanotechnology and proteomic tools in crop development towards sustainable agriculture
    (Springer, 2024) Daniel, Augustine Innalegwu; Hüsselmann, Lizex; Shittu, Oluwatosin Kudirat; Gokul, Arun
    The increase in global population which translates to increased demand for food called for urgent attention from key players and policy makers in agricultural sector. Also, the effects of climate change and its consequent biotic and abiotic stresses in plants has greatly affect the sustainability of agriculture and production of food. These challenges require novel and sustainable approaches to improve the quality and yield of crops. The vast application of nanoparticles in different fields of study is attributed to their distinct chemical and physical characteristics. However, in agriculture, their application is limited because of their safety concern. Currently, research is tailored to study the response of plants to various nanoparticle treatments; however, these studies are inconclusive due to limited knowledge on the mechanisms of plant–nanoparticle interactions. Recently, studies on nanobiotechnology have taken a new dimension from preliminary bioassay experiments to more complex, research-oriented studies using various omics tools. Changes in protein expression caused by plant–nanoparticle interaction at any developmental stages, or tissue types may be investigated using suitable proteomics techniques. This review discussed the various applications of nanotechnology and proteomic tools in plant growth and development. Plant–nanoparticle interactions and the application of nanoparticles and proteomic tools in genetic engineering of plants to attain agricultural sustainability and food safety are also discussed. Informative and thorough understanding of plant–nanoparticle interaction will serve as a blueprint that will enable plant scientist and genetic engineers to develop plant biomarkers and explore their potential application for crop improvement.
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    Biocontrol Potential of Bacillus subtilis and Bacillus tequilensis against Four Fusarium Species
    (MDPI, 2023) Baard, Vejonepher; Bakare, Olalekan Olanrewaju; Daniel, Augustine Innalegwu; Nkomo, Mbukeni; Gokul, Arun
    The use of biological control agents as opposed to synthetic agrochemicals to control plant pathogens has gained momentum, considering their numerous advantages. The aim of this study is to investigate the biocontrol potential of plant bacterial isolates against Fusarium oxysporum, Fusarium proliferatum, Fusarium culmorum, and Fusarium verticillioides. Isolation, identification, characterization, and in vitro biocontrol antagonistic assays of these isolates against Fusarium species were carried out following standard protocols. The bacterial endophytes were isolated from Glycine max. L leaves (B1), Brassica napus. L seeds (B2), Vigna unguiculata seeds (B3), and Glycine max. L seeds (B4). The bacterial isolates were identified using 16S rRNA PCR sequencing. A phylogenetic analysis shows that the bacterial isolates are closely related to Bacillus subtilis (B1) and Bacillus tequilensis (B2–B4), with an identity score above 98%. All the bacterial isolates produced a significant amount (p < 0.05) of indole acetic acid (IAA), siderophores, and protease activity. In vitro antagonistic assays of these isolates show a significant (p < 0.05) growth inhibition of the fungal mycelia in the following order: F. proliferatum > F. culmorum > F. verticillioides > F. oxysporum, compared to the control. The results suggest that these bacterial isolates are good biocontrol candidates against the selected Fusarium species
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    Hypoglycaemic activity of biosynthesized copper oxide nanoparticles in alloxan-induced diabetic Wister rats
    (Wiley, 2023) Umar, Maimuna Bello; Daniel, Augustine Innalegwu; Tijani, Jimoh Oladejo
    Diabetes mellitus (DM) is a metabolic disorder that affects the body's ability to produce or use insulin. This study evaluated the hypoglycaemic activity of biosynthesized copper oxide nanoparticles (CuO-NPs) in alloxan-induced diabetic Wister rats. CuO-NPs were synthesized via the green route and characterized using different analytical tools. Diabetes was induced intraperitoneally using 90 mg/kg body weight of alloxan monohydrate in albino rats. Thirty (30) rats were randomly divided into 5 groups of 6 rats each and orally treated for 21 days. Groups I and II were treated with 300 mg/kg bwt Cereus hildmannianus extract and CuO-NPs, respectively. Groups III and IV received 5 mg/kg bwt of Glibenclamide and 2 mL of normal saline, respectively, while Group V was left untreated as the diabetic control. Blood glucose (BG) levels and body weight changes were monitored at 3- and 7- day intervals, respectively, throughout 21-day treatment period. Lipid profiles, enzyme assays and histopathological studies of the liver were also carried out.