Browsing by Author "Bapoo, Rafik"
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Item FTIR, dissolution and anti-viral activity of nevirapine co-crystals(OMICS International, 2017) Samsodien, Halima; Bapoo, Rafik; Doms, T.I.; Harneker, Z.; Louw, A.S.; Scheepers, I.C.; Sonday, A.B.; Geldenhuys, B.The study uses Fourier Transform Infrared (FTIR) spectroscopy to identify five Nevirapine (NV) co-crystals, determines the dissolution profile of the co-crystals and the antiviral activity comparative to pure NV. Hot stage microscopy measured the purity and integrity of each co-crystal. FTIR analysis was used to identify the co-crystals to make recommendations regarding the future use of the technique to identify the NV co-crystals. Dissolution studies of the NV co-crystals prepared with maleic acid, salicylic acid and glutaric acid (NVMLE, NVSLI and NVGLT, respectively) were completed using the rotating basket method. Assays were conducted using High Performance Liquid Chromatography and compared to pure NV and the five NV: co-former mixtures. The antiviral activity was tested to determine whether the co-crystals had an improved activity against HIV-1 compared to pure NV. All co-crystals, except NVTTA (a NV co-crystal prepared with rac-tartaric acid), were pure and maintained their integrity for approximately one year. NVGLT, NVMLE and NVTTA, 1:1 molar ratio co-crystals were identified by FTIR. The C=O stretching frequency of the carboxylic acid groups of NV and GLT were observed at 1638.15 cm-1 and 1719.23 cm-1 in the NVGLT co-crystal which corresponded with spectra of NVMLE and NVTTA. In NVMLE the C=O stretching frequency of the C=O of NV and MLE were observed at 1640.58 cm-1 and 1694.10 cm-1 and in NVTTA it was at 1637.25 cm-1 and 1708.50 cm-1, suggesting the presence of both parent molecules in the new phase for NVGLT, NVMLE and NVTTA. Dissolution studies suggested that NVGLT was the only co-crystal that yielded better results than both NV and its physical mixture. The antiviral activity of the NVSC (an NV co-crystal prepared with saccharin) and NVSLI cocrystals in DMSO was significantly different to pure NV, demonstrating an improvement in anti-viral activity.Item Pharmaceutical formulation and in-vitro testing of Dioxy MP 14 (stabilised chlorine dioxide) against mycobacteria tuberculosis(University of the Western Cape, 2011) Mavu, Daniel Muleya; Uebel, Reinhard; Bapoo, Rafik; Hayward, DonThis study was based on Dioxy MP 14 (DMP), a brand of stabilized chlorine dioxide (SCD). The active pharmaceutical ingredient (API) of DMP is chlorine dioxide (CD) which is a potent oxidant and biocide. These properties have proved invaluable for various applications. The main goals of this study were: to evaluate the effectiveness of DMP for disinfecting Mycobateria tuberculosis (TB) contaminated medical instruments, devices, floors and surfaces; to investigate the stability of DMP; and to explore possibilities for medical application of DMP. Evaluation of disinfectant activity of DMP on TB was performed using the spectrophotometric method, a modification of the European suspension test, EN 14348. M. bovis BCG was employed as surrogate in this test. Results were as follows: The minimum inhibitory concentration (MIC₉₀) = 12.5 ppm; the minimum bactericidal concentration (MBC) = 15.4 ppm; the Mycobactericidal Effect (ME) = 8.8log reduction; and the minimum inhibitory concentration (MIC₉₀) x minimum exposure time (CT) = 12.5 ppm.s. The long term stability study of DMP was performed by monitoring the rate of degradation of DMP stored in the fridge (2-8 °C), in the oven (40 °C), and under ambient conditions (15-30 °C). Analytical methods of assessing DMP concentration was by Iodometric titration method. The shelf life of DMP stored in a transparent bottle at room temperature was 9.8 weeks, as opposed to 52.7 weeks when stored in an amber colored reagent bottle at the same temperature. Both oven samples had an expiry date of about 20 weeks and the fridge samples about 70 weeks. Foam formulations for a vaginal douche (VGD), mouth rinse (MRF), and foot/sit bubble bath (F/SBB)], were developed in the laboratory. DMP and the formulated concentrate were designed to be mixed just prior to administration. During foam evaluation studies, a mechanical overhead stirrer was used to generate foam. Foamability was assessed by quantifying the amount of foam generated. The stability of foams were assessed by: 1) determining the rate of foam decay and the rate of foam drainage observed concurrently from foam loaded in a measuring cylinder; and 2) determining the life span of single bubbles of each foam system i.e. the bubble breaking time (BBT). The density of each foam system was also determined. Potentiometric acid base titration was used to select suitable adjuster alkali, and to show the benefits of employing a buffer. Concentrate development was initiated by a simple mixture of all the ingredients followed by stirring and observing the deviations from desired quality attributes of the product. The subsequent five processes were improvements designed to circumvent the shortcomings of the initial procedure to arrive at the optimized method E. Prototype formulations were employed to optimize excipient quantities to eventually arrive at an optimized master formula. In foam evaluation, it was found that sodium lauryl sulphate/ammonium lauryl sulphate/cocoamidopropyl betaine/cetostearyl alcohol (SLS/ALS/CAPB/CSA) foam system was the most appropriate to use in the formulation. NaOH was selected as the adjuster solution and KHP as the buffer. The dosage formula (DF) of the VGD and F/SBB was determined to be MDF = 5 ml of 50 ppm DMP + 5 ml concentrate + 40 ml water = 50 ml and that of MRF as MDF = 19 ml diluted concentrate + 1 ml of 50 ppm DMP. In conclusion, DMP was found to be a highly effective disinfectant against Mycobacteria. DMP has reasonable shelf life if stored appropriately. Pharmaceutical formulation from DMP was found to be delicate due to the narrow pH window of DMP stability, but is feasible.Item Service learning in pharmacy: Opportunities for student learning and service delivery(Academic Journals, 2011) Bheekie, Angeni; Obikeze, Kenechukwu; Bapoo, Rafik; Ebrahim, NaushaadHigh patient load and understaffing in public health care facilities preclude the provision of optimal pharmaceutical services in South Africa. A Service Learning in Pharmacy (SLIP) programme for the University of the Western Cape's final year pharmacy students was implemented in health care facilities to assist in service provision. Students rotated between a pharmacotherapy (patient-oriented) and pharmaceutical formulation (product-oriented) activities to develop skills in prescription analysis, manufacturing and packaging of hospital pharmaceuticals. Structured focus group sessions were held with students and pharmacists to assess the integrated service learning experiences. Student feedback was positive, as the 'real world' experiences enabled them to adapt to intense work pressures, developed a sense of 'personal responsibility' towards patient health and they were sensitized to issues of social injustice. Students became competent in prescription analyses, counseling on medication use, manufacturing and pre-packing procedures. Pharmacists fully supported increased student involvement in the health services. Service learning in pharmacy schools is needed to contextualize learning and to address health care needs in South Africa.