Research Articles (School of Pharmacy)

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Browsing by Author "Amabeoku, G.J."

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    Evaluation of the antidiarrhoeal and antidiabetic activities of the leaf aqueous extract of Syzygium cordatum hoscht. ex C.Krauss (Mytraceae) in rodents
    (Asian Network for Scientific Information, 2013) Deliwe, M.; Amabeoku, G.J.
    Syzygium cordatum Hoscht. ex C.Krauss is widely used by traditional medicine practitioners to treat many ailments including diarrhoea and diabetes. Despite the folklore use, little evidence can be found in literature to corroborate the claims of therapeutic success of the plant species. The objective of the study was to investigate the antidiarrhoeal and antidiabetic activities of the leaf aqueous extract of the plant species in mice and rats, respectively. The antidiarrhoeal activity of the leaf aqueous extract of S. cordatum was investigated using castor oil-induced diarrhoeal test. The antidiabetic activity of the plant extract was studied using streptozotoxin-induced diabetes in rats. Acute toxicity study of plant extract was also carried out using a standard method. Leaf aqueous extract of S. cordatum significantly reduced the number of diarrhoeal episodes, decreased the stool mass and delayed the onset of castor oil-induced diarrhoea in mice. Loperamide was shown to protect the animals against castor oil-induced diarrhoea. Both the leaf aqueous extract of S. cordatum and chlorpropamide significantly lowered the blood glucose levels in both normal and streptozotoxin-induced diabetic rats. The LD50 value obtained for the plant extract was over 4000 mg kg-1 orally. The results obtained suggest that the leaf aqueous extract of S. cordatum has both antidiarrhoeal and antidiabetic activities. This justifies the folklore use of the plant species by traditional medicine practitioners to treat diarrhoea and diabetes. The relatively high LD50 value obtained for the leaf aqueous extract shows that the plant species is non toxic to mice.
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    Involvement of gamma aminobutyric acid in the anticonvulsant effect of the leaf methanol extract of Ruta graveolens L. (Rutaceae) in mice
    (Asian Network for Scientific Information, 2013) Ahmad, A.H.; Amabeoku, G.J.
    The possible involvement of gamma aminobutyric acid (GABA), in the anticonvulsant effect of Ruta graveolens L. was investigated by studying the effect of the leaf methanol extract against seizures elicited by either pentylenetetrazole (PTZ), bicuculline, picrotoxin or N-Methyl-DL-Aspartic acid (NlvIDLA) in mice. Leaf methanol extract of Ruta graveolens, phenobarbitone, diazepam and muscimol significantly antagonized seizures induced by PTZ, bicuculline or picrotoxin. Combined treatment of subeffective doses of R. graveolens and muscimol significantly antagonized seizures induced by PTZ, bicuculline or picrotoxin. Dimethylsulfoxide (DMSO) or phenytoin did not significantly affect the seizures produced by PTZ, bicuculline or picrotoxin. Ruta graveolens, phenobarbitone, diazepam, phenytoin or DMSO did not significantly affect seizures produced by NlvIDLA. LY233053 significantly antagonized seizures produced by NlvIDLA. Combined treatment of sub-effective doses of LY233053 and Ruta graveolens did not significantly alter NlvIDLA-induced seizures. The phytochemical qualitative analysis of the plant species showed the presence of tannins, cardiac glycosides, saponins, flavonoids, triterpene steroids and alkaloids. The LD50 value obtained following oral administration of the leaf methanol extract of R. graveolens was above 4000 mg kg-1 .The HPLC fingerprint of the plant species revealed certain characteristic peaks at 350 run. The data obtained in this study, indicate that the leaf methanol extract of R. graveolens has anticonvulsant activity. The data obtained also indicate that GABA mechanism may probably be involved in the anticonvulsant effect of the plant extract. The relatively high LD50 obtained for the plant species, given orally, indicates that it is safe in mice.