Comparative analysis of tests used to assess sperm chromatin integrity and DNA fragmentation

Simple item page
dc.contributor.authorHenkel, R
dc.contributor.authorDutta, S
dc.contributor.authorAgarwal, A
dc.date.accessioned2021-05-11T12:58:44Z
dc.date.available2021-05-11T12:58:44Z
dc.date.issued2021
dc.description.abstractMale infertility has a complex etiology, and many times, the cause is unknown. While routine semen analysis provides an overview of basic semen parameters, such as sperm concentration, motility, viability and morphology, a significant overlap of these parameters has been reported in fertile and infertile men. Moreover, conventional semen parameters do not reveal the cellular or molecular mechanisms of sperm dysfunctions leading to infertility. Therefore, sperm functional parameters, including sperm chromatin integrity, are evaluated to provide information on subtle sperm defects that are not routinely identified. Incomplete or defective sperm chromatin condensation increases the susceptibility of the sperm DNA to oxidative damage or other factors. To evaluate sperm chromatin integrity, different methods with varying degrees of diagnostic and prognostic capabilities are available. Among these assays, SCSA, TUNEL and SCD assays are most commonly used. While these assays rather evaluate the DNA directly for damages, the aniline blue and chromomycin A3 stains test for the quality of chromatin condensation.en_US
dc.identifier.citationHenkel, R. et al. (2021). Comparative analysis of tests used to assess sperm chromatin integrity and DNA fragmentation. Andrologia, 53(2),e13718en_US
dc.identifier.issn0303-4569
dc.identifier.uri10.1111/and.13718
dc.identifier.urihttp://hdl.handle.net/10566/6147
dc.language.isoenen_US
dc.publisherBlackwell Publishing Ltden_US
dc.subjectART outcomesen_US
dc.subjectMale infertilityen_US
dc.subjectOxidative stressen_US
dc.subjectsperm DNA fragmentationen_US
dc.subjectTUNEL assayen_US
dc.titleComparative analysis of tests used to assess sperm chromatin integrity and DNA fragmentationen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
and.13718.pdf
Size:
879.82 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Research Articles (Medical Bioscience)