Effects of temperature and storage time on the motility, viability, DNA integrity and apoptosis of processed human spermatozoa

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dc.contributor.authorIranpour, Farhad Golshan
dc.contributor.authorNateghian, Zohre
dc.contributor.authorHenkel, Ralf
dc.date.accessioned2023-06-07T07:36:05Z
dc.date.available2023-06-07T07:36:05Z
dc.date.issued2019
dc.description.abstractThe aim of this study was to evaluate motility, viability, DNA integrity and apoptosis of spermatozoa when washed semen samples were kept for up to 12 days at 4–6°C and 25°C. In this experimental study, 26 normozoospermic semen samples were washed twice in Modified Ham's F10 and resuspended in IVF fertilisation medium. Half of the specimens were stored at 4–6°C, and the other half was kept at 25°C for 12 days. The proportions of viable, motile, spermatozoa with double-stranded DNA and apoptotic spermatozoa were examined during storage time. Apoptosis was measured using annexin V-PI staining followed by flow cytometry. Results showed that sperm motility and viability decreased during 12 days of sample storage (p < .001). There was no significant difference between the two temperatures in terms of motility and viability for up to 2 days (p < .05).en_US
dc.identifier.citationIranpour, F. G. et al. (2019). Effects of temperature and storage time on the motility, viability, DNA integrity and apoptosis of processed human spermatozoa. Andrologia, 52 (2). https://doi.org/10.1111/and.13485en_US
dc.identifier.issn1439-0272
dc.identifier.urihttps://doi.org/10.1111/and.13485
dc.identifier.urihttp://hdl.handle.net/10566/9049
dc.language.isoenen_US
dc.publisherWileyen_US
dc.subjectBioscienceen_US
dc.subjectDNA fragmentationen_US
dc.subjectSperm motilityen_US
dc.subjectHuman geneticsen_US
dc.subjectMedical Sciencesen_US
dc.titleEffects of temperature and storage time on the motility, viability, DNA integrity and apoptosis of processed human spermatozoaen_US
dc.typeArticleen_US

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