Molecular characterization of the 16S rRNA Gene of helicobacter fennelliae isolated from stools and blood cultures from paediatric patients in South Africa

Simple item page
dc.contributor.authorSmuts, H.E.M
dc.contributor.authorLastovica, Albert J.
dc.date.accessioned2016-06-08T12:16:58Z
dc.date.available2016-06-08T12:16:58Z
dc.date.issued2011
dc.description.abstractForty strains of H. fennelliae collected from paediatric blood and stool samples over an 18 year period at a children's hospital in Cape Town, South Africa, were amplified by PCR of the 16S rRNA. Two distinct genotypes of H. fennelliae were identified based on the phylogenetic analysis. This was confirmed by sequencing a portion of the beta subunit of the RNA polymerase (rpoB) gene. All isolates from South Africa clustered with a proposed novel Helicobacter strain (accession number AF237612) isolated in Australia, while three H. fennelliae type strains from the northern hemisphere, NCTC 11612, LMG 7546 and CCUG 18820, formed a separate branch. A large (355bp) highly conserved intervening sequence (IVS) in the 16S rRNA was found in all isolates. Predicted secondary structures of the IVS from the 16S rRNA and 23S rRNA were characterised by a primary stem structure formed by base pairing of the 3 and 5 ends and internal loops and stems. This phylogenetic analysis is the largest undertaken of H. fennelliae. The South African H. fennelliae isolates are closely related to an Australian isolate previously reported to be a possible novel species of Helicobacter. This study suggests that the latter is strain of H. fennelliae.en_US
dc.identifier.citationSmuts, H.E.M. and Lastovica, A.J. (2011). Molecular characterization of the 16S rRNA Gene of helicobacter fennelliae isolated from stools and blood cultures from paediatric patients in South Africa. Journal of Pathogens, Article ID 217376en_US
dc.identifier.issn2090-3057
dc.identifier.urihttp://hdl.handle.net/10566/2291
dc.language.isoenen_US
dc.privacy.showsubmitterFALSE
dc.publisherHindawien_US
dc.rights.uriCopyright 2011 H. E. M. Smuts and A. J. Lastovica. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.source.urihttp://dx.doi.org/10.4061/2011/217376
dc.status.ispeerreviewedTRUE
dc.subjectHelicobacter fennelliaeen_US
dc.subjectPaediatric blooden_US
dc.subjectSouth Africaen_US
dc.subjectPaediatric patientsen_US
dc.subjectHelicobacteren_US
dc.titleMolecular characterization of the 16S rRNA Gene of helicobacter fennelliae isolated from stools and blood cultures from paediatric patients in South Africaen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Smuts_Molecular_2011.pdf
Size:
1.74 MB
Format:
Adobe Portable Document Format
Description:
Download

Collections

Research Articles (IMBM)