Exogenous 3,3′-diindolylmethane improves vanadium stress tolerance in brassica napus seedling shoots by modulating antioxidant enzyme activities

dc.contributor.authorFahiem Carelse, M
dc.contributor.authorGokul, A
dc.contributor.authorNiekerk, L.-A
dc.date.accessioned2021-05-11T12:21:44Z
dc.date.available2021-05-11T12:21:44Z
dc.date.issued2021
dc.description.abstract3,3′-diindolylmethane (DIM) belongs to a family of indole glucosinolate compounds that have been shown to improve Brassica napus growth through the modulation of reactive oxygen species when applied exogenously. The B. napus cultivar AV Garnet was previously identified as a vanadium-sensitive cultivar. Therefore, in this study we investigated whether exogenous DIM could improve the vanadium tolerance of AV Garnet. We performed the following experiments: seed germination assessment, dry weight assessment, cell viability assay, chlorophyll content assay, mal-ondialdehyde (MDA) assay, conjugated diene (CD) content assay, hydrogen peroxide (H2O2 ) content assay, superoxide (O2−) content determination, methylglyoxal (MG) content determination, hydroxyl radical (·OH) concentration determination, ascorbate peroxidase (APX) activity assay, superoxide dismutase (SOD) activity assay, glyoxalase I (Gly I) activity assay, glutathione S-transferase (GST) activity assay and inductively coupled plasma optical emission spectroscopy (ICP-OES) analysis for vanadium content determination. Under vanadium stress, exogenous DIM increased the seed germination percentage, shoot dry weight, cell viability and chlorophyll content.en_US
dc.identifier.citationFahiem Carelse, M. et al. (2021). Exogenous 3,3′-diindolylmethane improves vanadium stress tolerance in brassica napus seedling shoots by modulating antioxidant enzyme activities. Biomolecules, 11(3),436,1-18en_US
dc.identifier.issn2218-273X
dc.identifier.uri10.3390/biom11030436
dc.identifier.urihttp://hdl.handle.net/10566/6142
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subject3,3′-diindolylmethaneen_US
dc.subjectAntioxidant enzymesen_US
dc.subjectBrassica napusen_US
dc.subjectReactive oxygen speciesen_US
dc.subjectVanadiumen_US
dc.titleExogenous 3,3′-diindolylmethane improves vanadium stress tolerance in brassica napus seedling shoots by modulating antioxidant enzyme activitiesen_US
dc.typeArticleen_US

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