Calibration of redox potential in sperm wash media and evaluation of oxidation–reduction potential values in various assisted reproductive technology culture media using MiOXSYS system

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dc.contributor.authorPanner Selvam, M. K.
dc.contributor.authorHenkel, Ralf
dc.contributor.authorSharma, Rajan
dc.contributor.authorAgarwal, A.
dc.date.accessioned2018-01-17T11:30:05Z
dc.date.available2018-01-17T11:30:05Z
dc.date.issued2018
dc.description.abstractOxidation–reduction potential describes the balance between the oxidants and antioxidants in fluids including semen. Various artificial culture media are used in andrology and IVF laboratories for sperm preparation and to support the development of fertilized oocytes under in vitro conditions. The composition and conditions of these media are vital for optimal functioning of the gametes. Currently, there are no data on the status of redox potential of sperm processing and assisted reproduction media. The purpose of this study was to compare the oxidation–reduction potential values of the different media and to calibrate the oxidation–reduction potential values of the sperm wash medium using oxidative stress inducer cumene hydroperoxide and antioxidant ascorbic acid. Redox potential was measured in 10 different media ranging from sperm wash media, freezing media and assisted reproductive technology one-step medium to sequential media. Oxidation–reduction potential values of the sequential culture medium and one-step culture medium were lower and significantly different (p < 0.05) from the sperm wash media. Calibration of the sperm wash media using the oxidant cumene hydroperoxide and antioxidant ascorbic acid demonstrated that oxidation–reduction potential and the concentration of oxidant or antioxidant are logarithmically dependent. This study highlights the importance of calibrating the oxidation–reduction potential levels of the sperm wash media in order to utilize it as a reference value to identify the physiological range of oxidation oxidation–reduction potential that does not have any adverse effect on normal physiological sperm function.en_US
dc.identifier.citationSelvam, M.K.P. et al. (2018). Calibration of redox potential insperm wash media and evaluation of oxidation–reduction potentialvalues in various assistedreproductive technology culturemedia using MiOXSYS system. Andrology, 2018en_US
dc.identifier.issn2047-2919
dc.identifier.urihttp://dx.doi.org/10.1111/andr.12461
dc.identifier.urihttp://hdl.handle.net/10566/3394
dc.language.isoenen_US
dc.privacy.showsubmitterFALSE
dc.publisherWileyen_US
dc.rightsThis is the author-version of the article published online at: http://dx.doi.org/10.1111/andr.12461
dc.status.ispeerreviewedTRUE
dc.subjectAssisted reproductive technologyen_US
dc.subjectOne-step mediaen_US
dc.subjectOxidation–reduction potentialen_US
dc.subjectSequential mediaen_US
dc.subjectSperm wash mediaen_US
dc.titleCalibration of redox potential in sperm wash media and evaluation of oxidation–reduction potential values in various assisted reproductive technology culture media using MiOXSYS systemen_US
dc.typeArticleen_US

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